Mannotriose regulates learning and memory signal transduction in the hippocampus  

作　　者：Lina Zhang Weiwei Dai Xueli Zhang Zhangbin Gong Guoqin Jin 

机构地区：[1]Department of Biochemistry,Institute of Basic Medicine,Shanghai University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2013年第32期3020-3026,共7页中国神经再生研究（英文版）

基　　金：financially supported by the National Natural Science Foundation of China,No.30873317;the Leading Academic Discipline Project of Shanghai Municipal Education Commission,No.J50301;"085 Class Disciplines Building"Scientific and Technology Innovation Fundation,No.085ZY1204;Shanghai Health Bureau of Scientific Research Projects,No.20124Y025

摘　　要：Rehmannia is a commonly used Chinese herb, which improves ieaming and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Pri- mary hippocampal neurons were cultured in vitro, insulted with high-concentration （1 × 10-4 mol/L） cor- ticosterone, and treated with 1 × 104 mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorti- cold receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were all dramatically decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.Rehmannia is a commonly used Chinese herb, which improves ieaming and memory. However, the crucial components of the signal transduction pathway associated with this effect remain elusive. Pri- mary hippocampal neurons were cultured in vitro, insulted with high-concentration （1 × 10-4 mol/L） cor- ticosterone, and treated with 1 × 104 mol/L mannotriose. Thiazolyl blue tetrazolium bromide assay and western blot analysis showed that hippocampal neuron survival rates and protein levels of glucocorti- cold receptor, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor were all dramatically decreased after high-concentration corticosterone-induced injury. This effect was reversed by mannotriose, to a similar level as RU38486 and donepezil. Our findings indicate that mannotriose could protect hippocampal neurons from high-concentration corticosterone-induced injury. The mechanism by which this occurred was associated with levels of glucocorticoid receptor protein, serum and glucocorticoid-regulated protein kinase, and brain-derived neurotrophic factor.

关 键 词：neural regeneration traditional Chinese medicine REHMANNIA mannotriose CORTICOSTERONE HIPPOCAMPUS neurons learning and memory grants-supported paper NEUROREGENERATION 

分 类 号：R338[医药卫生—人体生理学]