A magnetic nanoparticles-based method for DNA extraction from the saliva of stroke patients  

作　　者：Li Yi Ying Huang Ting Wu Jun Wu 

机构地区：[1]Department of Neurology,Peking University Shenzhen Hospital

出　　处：《Neural Regeneration Research》2013年第32期3036-3046,共11页中国神经再生研究（英文版）

基　　金：supported by the Scientific and Technological Innovation Foundation of Shenzhen,China,No.JC201105180757;Shenzhen Science and Technology Projects,No.201002063

摘　　要：C677T polymorphism in the methylenetetrahydrofolate reductase （MTHFR） gene is a risk factor for stroke, suggesting that widespread detection could help to prevent stroke. DNA from 70 stroke pa- tients and 70 healthy controls was extracted from saliva using a magnetic nanoparticles-based method and from blood using conventional methods. Real-time PCR results revealed that the C677T polymorphism was genotyped by PCR using DNA extracted from both saliva and blood samples. The genotype results were confirmed by gene sequencing, and results for saliva and blood samples were consistent. The mutation TT genotype frequency was significantly higher in the stroke group than in controls. Homocysteine levels were significantly higher than controls in both TT genotype groups. Therefore, this noninvasive magnetic nanoparticles-based method using saliva samples could be used to screen for the MTHFR C677T polymorphism in target populations.C677T polymorphism in the methylenetetrahydrofolate reductase （MTHFR） gene is a risk factor for stroke, suggesting that widespread detection could help to prevent stroke. DNA from 70 stroke pa- tients and 70 healthy controls was extracted from saliva using a magnetic nanoparticles-based method and from blood using conventional methods. Real-time PCR results revealed that the C677T polymorphism was genotyped by PCR using DNA extracted from both saliva and blood samples. The genotype results were confirmed by gene sequencing, and results for saliva and blood samples were consistent. The mutation TT genotype frequency was significantly higher in the stroke group than in controls. Homocysteine levels were significantly higher than controls in both TT genotype groups. Therefore, this noninvasive magnetic nanoparticles-based method using saliva samples could be used to screen for the MTHFR C677T polymorphism in target populations.

关 键 词：neural regeneration brain injury stroke magnetic nanoparticles SALIVA methylenetetrahydrofolatereductase HOMOCYSTEINE gene polymorphism gene screening grants-supported paper NEUROREGENERATION 

分 类 号：R743.3[医药卫生—神经病学与精神病学]