长白山野猪与杜洛克猪杂交1代猪肌内脂肪代谢酶HSL和MDH活性的发育性变化规律及其对肌内脂肪沉积影响的研究  

The Development Changes of HSL and MDH Activities in Muscle and Their Association with Intramuscular Fat in Mount Changbai Boar×Duroc Pigs

作　　者：王倩[1] 尹逊河[1] 王元虎 李少青 王宪龙[1] 祝仁铸[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东绿涧生态产业园,山东章丘250200

出　　处：《中国畜牧兽医》2013年第12期121-124,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山东省科技攻关项目(2010GNC10930)

摘　　要：为了研究长白山野猪与杜洛克猪杂交1代猪肌内脂肪代谢酶激素敏感脂酶(hormone-sensitive lipase,HSL)和苹果酸脱氢酶(malate dehydrogenase,MDH)活性的发育性变化规律及其对肌内脂肪沉积的影响,试验以5个体重组(20、35、50、70和90kg)的长白山野猪与杜洛克猪的杂交1代猪(简称野杜F1代猪,各40头,公、母各半)为研究对象,研究了肌肉组织中脂肪代谢酶活性的发育性变化规律与肌内脂肪含量和肌肉嫩度的关系。结果表明,在生长期随着体重的增加,肌肉组织中HSL和MDH的活性差异显著(P<0.05),野杜F1代猪HSL活性均高于莱芜黑猪的活性(90kg除外),且在35kg时,野杜F1代猪和莱芜黑猪差异极显著(P<0.01);肌肉组织中的MDH活性变化,野杜F1代猪呈先升后降的趋势,活性最高点在70kg;在90kg时野杜F1代猪中MDH活性极显著低于莱芜黑猪(P<0.01)。不同体重阶段,肌肉嫩度差异显著(P<0.05),且随着体重的增加而呈不断升高的趋势。相关分析结果表明,野杜F1代猪90kg时肌肉组织中MDH活性与肌内脂肪含量呈极显著正相关(r=0.97,P<0.01),HSL和MDH的活性与肌肉嫩度呈显著正相关(P<0.05)。研究结果提示,肌肉组织中的HSL和MDH对肌内脂肪的沉积量有一定的影响,可以通过控制其活性调节肌内脂肪的含量,且肌内脂肪的沉积量与肌肉嫩度有一定的联系。This experiment was conducted to study the development changes of fatty enzymatic hormone-sensitive lipase （HSL） and malate dehydrogenase （MDH） activities and their association with intramuscular fat in F1 hybrids with Mount Changbai Boar and Duroc pigs. To investigate the development changes of fatty enzymatic activities and their association with intramuscular fat and meat tenderness when the body weight got 20,35,50,70 and 90 kg levels F1 hybrids with boar and Laiwu pigs and white pigs of Landrace and Yorkshire （each 40 heads and male, female in the same evenly）. The results indicated that with gaining of body weight, the activity of HSL and MDH were significantly different in muscle （P〈0.05）. The activity of HSL in F1 hybrids with Mount Changbai boar and Duroc pigs was significantly higher than Laiwu pigs at 35 kg （P〈0.01）; the activities of MDH in muscle in F1 hybrids was increased firstly and then decreased, and reached a peak at 70 kg of weight. The activity of MDH in F1 hybrids with Mount Changbai boar and Duroc pigs was significantly lower than Laiwu pigs at 90 kg （P〈0.01）. Correlation analysis showed that the activities of MDH in muscle were highly correlated to IMF content of F1 hybrids with Mount Changbai boar and Duroc pigs at 90 kg （r=0.97,P〈0.01）, the activities of HSL and MDH in muscle were highly correlated to the meat tenderness （P〈0.01）. The results suggested that the depositions of IMF could be improved by controlling the activity of fatty enzymatic HSL and MDH in muscle and there was some immanent contact between the depositions of IMF and the meat tenderness.

关 键 词：长白山野猪 杜洛克猪 激素敏感脂酶 苹果酸脱氢酶 肌内脂肪 肌肉嫩度 

分 类 号：S828[农业科学—畜牧学]