吴茱萸、甘草浸提液对亚硝化反应清除及阻断作用的对比研究  

Study on Scavenging and Disconnecting Effect of Nitrosation Reaction with Extracts of Tetradium Ruticarpum and Glycyrrhizae

作　　者：张成[1] 董侯燕 李爽[1] 曲悠扬[1] 蔡曙洁 杨虹[1] 康蔓妮 陶旻[1] 简惠美 颜亨梅[1] 

机构地区：[1]北京师范大学珠海分校工程技术学院生物工程系,广东珠海519087

出　　处：《中国农学通报》2013年第32期230-237,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"基于分子生物技术的蜘蛛摄食生态学分析及控虫效能评价"(31372159);北京师范大学珠海分校青年教师科研项目"药用植物吴茱萸;刺五加中黄酮含量提取及清除自由基和抗亚硝化作用研究"(201353015);北京师范大学珠海分校大学生创新创业训练计划"葡萄籽提取物的抗亚硝化作用研究"

摘　　要：为考查吴茱萸和甘草的浸提液对亚硝酸钠清除作用和对N-亚硝基化合物合成的阻断作用,及其两者作用效果比较,采用L9(33)正交试验法浸提活性成分,并测定对亚硝酸钠的清除率以及对N-亚硝基化合物合成的阻断率,确定最佳浸提工艺条件。以亚硝酸钠清除率为考察指标,吴茱萸最佳浸提工艺下对亚硝酸钠最大清除率为0.8365;而甘草最佳浸提工艺条件下最大清除率为0.5580。以对N-亚硝基化合物合成的阻断率为考察指标,吴茱萸最佳浸提工艺条件下对N-亚硝基化合物生成最大阻断率为0.9228;而甘草最佳浸提工艺条件下最大阻断率为0.6256。结果显示无论以对亚硝酸钠清除率为考察指标,还是以对N-亚硝基化合物合成的阻断率为考察指标,吴茱萸对亚硝化反应的抑制作用均强于甘草。In order to investigate and compare the effect of scavenging sodium nitrite and inhibit N-nitroso compounds formation which was influenced by the extracts of Tetradium ruticarpum and Glycyrrhizae, L（33） orthogonal test was chosen to extract the active components. In addition, the capacities of scavenging sodium nitrite and of inhibiting the N-nitroso compounds formation were measured to confirm the optimum condition of extracting. When the scavenging rate was considered as the main index, the maximum capacities of scavenging sodium nitrite by the extracts of Tetradium ruticarpum and Glycyrrhizae were 0.8365 and 0.5580, respectively. Furthermore, when the disconnection rate was considered as the main index, the maximum capacities of inhibiting N-nitroso compounds formation by the extracts of Tetradium rutiearpum and Glyeyrrhizae were 0.9228 and 0.6256, respectively. As a result, the capacity of inhibiting nitrosation reaction by Tetradium rutiearpum was shown to be much stronger than Glyeyrrhizae, no matter the scavenging rate or the disconnection rate as the main index to be considered.

关 键 词：吴茱萸、甘草浸提液 阻断 清除 亚硝酸钠 N-亚硝基化合物 

分 类 号：O623.54[理学—有机化学] Q946[理学—化学]