高效液相色谱法测定辣椒制品中8种工业染料的方法研究  

Determination method of 8 industrial dyes in chilli products by high performance liquid chromatography

作　　者：梁桂娟[1] 李红洲[1] 王文平[2] 孙棣[1] 肖洋[1] 张建[1] 

机构地区：[1]贵州省产品质量监督检验院,贵阳550003 [2]贵州大学化学工程学院,贵阳550025

出　　处：《食品科技》2013年第12期329-333,共5页Food Science and Technology

基　　金：国家质检总局项目(2012QK385)

摘　　要：目的：建立准确简便测定辣椒制品中多种非法添加工业染料的检测方法。方法：以95％丙酮水溶液为提取溶剂，采用O．05mol／L乙酸铵（pH4．5）：甲醇的流动相体系，用高效液相色谱法同时测定8种工业染料。结果：8种工业染料在各自的线性范围内呈现良好的线性，R2均大于0．99，定性检出限为0．017～0．073mg／L，定量检出限为0．28～1．20mg／kg，加标回收率在70％～104％之间，RSD在1．5～5．3％之间。结论：该方法简便灵敏，准确快速，可用于辣椒制品中8种工业染料的测定。Simulated reactor pressure vessel （RPV） model steel samples were heat treated at 880 ℃ for 0. 5 h and then water quenched and tempered at 660 ℃ for 10 h and finally aged at 300 -400 ℃for different time up to 12000 h. The precipitation of Cu-rich clusters in the simulated RPV model steel was investigated by means of atom probe tomography （APT） and hardness test. The results obtained by APT analysis show that the number density of Cu-rich clusters reaches 1.69 × 1023 m^-3 in the quenched specimen aged at 400 ℃ for 100 h. However, the number density of Cu-rich clusters merely reaches 6 × 1022m^- 3 in the quenched and tempered specimen aged at 400 ℃ for 1000 h. The results show that aging after quenching is a better process for simulation of Cu-rich clusters formation of RPV steel than the process of aging after quenching and tempering.

关 键 词：高效液相色谱 辣椒制品 工业染料 

分 类 号：TS207.5[轻工技术与工程—食品科学]