原生质体诱变选育高产谷胱甘肽菌株及基因表达分析  

Mutation of Saccharomyces cerevisiae Protoplasts for Enhanced Production of GSH and Gene Expression of GSH Over-accumulating Mutants

作　　者：孙姜[1] 朱益波[2] 王立梅[2] 邓大庆[2] 齐斌[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118 [2]常熟理工学院发酵工程技术研究中心,苏州食品生物技术重点实验室,江苏常熟215500

出　　处：《食品科学》2013年第23期176-179,共4页Food Science

基　　金：国家自然科学基金项目(31171758);江苏省高校"青蓝工程"中青年学术带头人项目;苏州市科技发展计划项目(SZS201007)

摘　　要：以Saccharomyces cerevisiaeY518为出发菌株,通过原生质体诱变提高产谷胱甘肽的能力。结果表明:用0.03mol/L HNO2处理原生质体3min,致死率为85.96%,突变率为6.85%;经过4轮连续的HNO2诱变,筛选得到一株胞内谷胱甘肽产量(14.85mg/g干质量)约比出发菌株高24%的突变株。进一步研究谷胱甘肽合成相关的基因表达量,发现其合成途径编码限速酶的基因GSH1的表达量上调了2.26倍。因此,突变株胞内谷胱甘肽水平的提高可能是由上调的GSH1的表达量导致的。In this study, Saccharomyces cerevisiae Y518 was improved for enhanced GSH production by protoplast mutagenesis. The mortality of Saccharomyces cerevisiae protoplasts after being treated with 0.03 mol/L HNO2 for 3 min was 85.96%. After 4 repeated treatments, a mutant was obtained which produced 14.85 mg of GSH per g dry weight, a 24% increase over the original strain. The gene GSH1 encoding the rate-limiting enzyme in the synthesis route showed a 2.26-fold increased expression, thus resulting in increased GSH production from the mutant strain.

关 键 词：SACCHAROMYCES CEREVISIAE 原生质体诱变 谷胱甘肽 基因GSH1 

分 类 号：Q815[生物学—生物工程]