纳米免疫磁珠富集单核增生李斯特菌  

Nanoparticle-based Immunomagnetic Separation of Listeria monocytogenes

作　　者：钟子清[1] 熊勇华[1] 赖卫华[1] 许恒毅[1] 山珊[1] 陈奇[1] 谢芳[1] 余扬帆[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2013年第23期212-215,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAK10B01);国家自然科学基金项目(31271863)

摘　　要：比较抗体直接偶联、链霉亲和素-生物素介导偶联的免疫磁珠以及免疫磁珠的粒径对单增李斯特菌捕获效率的影响。结果表明:链霉亲和素-生物素介导偶联的免疫磁珠捕获效率约是直接偶联法的3倍;同时,磁珠粒径对捕获效率也有较大影响,当免疫磁珠用量为100μg时,180、350、1150nm的免疫磁珠捕获效率分别为95%、18%和9%。因此,采用链霉亲和素-生物素介导偶联的180nm免疫磁珠是一种低成本、易操作、高效率的单核增生李斯特菌富集方法。The influence of antibody-coupling methods such as direct binding and streptavidin-biotin system on the capture efficiency of immunomagnetic beads of different sizes （180, 350 nm and 1150 nm） for Listeria monocytogenes was compared. The results showed that the capture efficiency of immunomagnetic beads using streptavidin-biotin method was approximately two-fold higher than that of direct binding method. Meanwhile, the size of magnetic beads had significantly effect on the capture efficiency. When the dosage of immunomagnetic beads was 100 μg, the capture efficiencies of beads 180, 350 nm and 1150 nm in diameter were 95%, 18% and 9%, respectively, during the separation of Listeria monocytogenes. Therefore, the 180-nm-diameter immunomagnetic beads prepared with streptavidin-biotin system can provide a low-cost, easy-to- use and efficient method for rapid enrichment and detection of Listeria monocytogenes.

关 键 词：单核增生李斯特菌 免疫磁分离 链霉亲和素 

分 类 号：TS201.3[轻工技术与工程—食品科学]