伏马菌素B_1对人正常食管上皮细胞细胞周期相关基因mRNA表达的影响  

Effect of Fumonisin B_1 on the mRNA Expressions of Cell Cycle-related Genes in Human Normal Esophageal Epithelial Cells

作　　者：王少康[1] 王婷婷[1] 黄桂玲[1] 石若夫[1] 杨立刚[1] 董淑楠[1] 孙桂菊[1] 

机构地区：[1]东南大学公共卫生学院营养与食品卫生学系,环境医学工程教育部重点实验室,江苏南京210009

出　　处：《食品科学》2013年第23期313-316,共4页Food Science

基　　金：国家自然科学基金项目(30800914;81372985);达能膳食营养研究与宣教基金项目(DIC2011-05);江苏省普通高校研究生科研创新计划项目(CXZZ-0179)

摘　　要：本研究采用体外实验,分离鉴定人正常食管上皮细胞,用RT-PCR方法测定伏马菌素B1(fumonisin B1,FB1)对细胞周期相关基因Cyclin D1、Cyclin E、P16、P21、P27mRNA表达的影响。结果表明:FB1作用人正常食管上皮细胞后可以在转录水平上影响Cyclin D1、Cyclin E、P16、P21、P27基因的表达,导致Cyclin D1mRNA高表达,Cyclin E、P16、P21、P27基因mRNA低表达。提示,细胞周期相关基因的异常表达与癌症密切相关,该研究从体外实验进一步证实FB1可能与人类食管癌的发生有关。Epidemiological studies have shown that fumonisin Bt （FB1）, a fungal toxin, may be associated with esophageal cancer, but the laboratory evidence is still limited. In this study, human normal esophageal epithelial cells were isolated and identified in vitro. The influence of FB1 on the mRNA expression of cell cycle-related genes including Cyclin D1, Cyclin E, P16, P21, and P27 was determined by reverse transcription-polymerase chain reaction （RT-PCR）. The results showed that FB1 played an important role in regulating the expression of Cyclin D1, Cyclin E, P16, P21, and P27 genes in normal human esophageal epithelial ceils at the transcriptional level, leading to high mRNA expression of Cyclin D1 but low mRNA expression of Cyclin E, P16, P21, and P27 genes. The abnormal expression of cell cycle-related genes is closely related with cancer. This in vitro study has further confirmed that FB1 is related with the occurrence of human esophageal cancer.

关 键 词：伏马菌素B1 人正常食管上皮细胞 细胞周期 基因表达 

分 类 号：R155.5[医药卫生—营养与食品卫生学]