液相色谱-质谱联用法检测马铃薯中α-茄碱含量  

Determination of α-solanine in Potato Using Liquid Chromatography-Mass Spectrometry

作　　者：伍慧敏[1] 曾静[2] 李美[2] 刘德明[2] 杜宇 熊兴耀[2] 曾建国[1,2,3] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湖南农业大学国家作物种质创新与资源利用重点实验室培育基地,湖南长沙410128 [3]湖南省中药提取工程研究中心,湖南长沙410331

出　　处：《食品科学》2013年第24期121-124,共4页Food Science

基　　金：国家现代农业产业技术体系建设专项(CARS-10-P19)

摘　　要：目的：建立基于液相色谱-质谱联用法的马铃薯中低含量的α-茄碱的高灵敏检测方法。方法：马铃薯用1％甲酸-甲醇（1：1，v／z）N声提取50min。采用C18色谱柱（2．1mm×150mm，5μm）分离，流动相为乙腈-0．1％甲酸（r／F），梯度洗脱，柱温35℃，流速0．2mL／min；采用液相色谱-电喷雾离子源单四极杆质谱检测。结果：α-茄碱在7～460μg／kg范围内与峰面积具有良好的线性关系，回归系数R^2=0．9991，平均加标回收率为93．6％，精密度为1．13％，重复性为2．59％。结论：该检测方法灵敏度高、重复性好，可用于马铃薯中低含量α-茄碱的含量测定。Objective： To establish a sensitive method to determine micro amounts of a-solanine in potato using liquid chromatograph-mass spectrometry （LC-MS）. Methods： Potato samples were extracted with 1% formic acid-methanol （1：1, V/V） in combination with ultrasonic assistance for 50 min. Separation of the extracts was performed on a C18 column （2.1 mm × 150 mm, 5 μm）. The mobile phase consisted of acetonitrile and 0.1% formic acid. Gradient elution was performed at column temperature of 35 ℃ with a flow rate of 0.2 mL/min. The pooled eluate was detected by electrospray ionization mass spectrometry. Results： The regression coefficient of a-solanine was 0.9991 in the range of 7-460 μg/kg. The average recovery rate was 93.6%. The precision as repeatability and reproducibility were 1.13% and 2.59%, respectively. Conclusion： This method is simple, accurate, repeatable and applicable to determine micro amounts of a-solanine in potato.

关 键 词：马铃薯 龙葵素 α-茄碱 液相色谱-质谱联用法 

分 类 号：S532[农业科学—作物学]