UPLC-Q-TOF-MS检测槲皮素代谢产物在大鼠血清及组织中的分布  

Determination of Quercetin Metabolites in Rats Serum and Tissues by UPLC-Q-TOF-MS

作　　者：邢萌萌[1] 朱科学[1] 聂少平[1] 李娜[1] 李文娟[1] 杨希[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2013年第24期158-162,共5页Food Science

基　　金：科技部国际合作项目(2010DFA31780)

摘　　要：目的:采用四极杆-飞行时间串联质谱(Q-TOF-MS)检测分析大鼠口服槲皮素单体后血清和组织中的代谢产物。方法:血清和组织样品采用Sephadex LH-20固相萃取小柱进行分离纯化,使用Q-TOF-MS检测,以体积分数0.1%甲酸溶液-乙腈为流动相梯度洗脱,电喷雾离子源负离子条件下进行扫描,鉴定大鼠给药槲皮素单体后血清及组织中的代谢产物。结果:槲皮素在血液中主要以其与葡糖醛酸、硫酸酯结合物的形式存在,约占全部代谢物的73.70%,一部分也以其与脂肪酸结合物的形式存在,约占26.30%;此外,槲皮素在体内分布广泛,在组织中的总含量由高到低依次为乳腺>子宫>卵巢>肾上腺>心>脾>肝>肾。结论:Q-TOF-MS可快速、有效地鉴定出SD大鼠血清及组织中的槲皮素代谢产物,为今后进行槲皮素与人类健康效应相关的研究提供参考。Objective： To determine quercetin metabolites in serum and tissues of rats after intragastric administration of quercetin aglycone by quadrupole-time of flight-mass spectrometry （Q-TOF-MS）. Methods： The pre-processed serum and tissue samples were separated and purified by Sephadex LH-20 solid-phase column. The collected aliquots were analyzed by Q-TOF-MS in the negative ion mode using gradient elution with a mobile phase composed of 0.1% formic acid （A） and acetonitrile （B） to identify the metabolites of quercetin. Results： The Q-TOF-MS analysis showed that quercetin was primarily metabolized to glucuronides and sulfates （73.70%） as well as esters （26.30%）. Moreover, quercetin and quercetin metabolites were widely distributed in rat tissues, especially in the female reproductive organs in SD rats. Its total concentration in tissues was as follows： breast 〉 uterus 〉 ovary 〉 adrenal gland 〉 heart 〉 spleen 〉 liver 〉 kidney. Conclusion： Q-TOF-MS can quickly and effectively identify quercetin metabolites in serum and tissues of SD rats and provide references for future ShldV on the relationship between quercetin or its metabolites and health effect in the future.

关 键 词：槲皮素 四极杆-飞行时间串联质谱仪 葡糖醛酸 硫酸酯 代谢物 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R969.1[医药卫生—公共卫生与预防医学]