Genetic Diversity of Tea Plant(Camellia Sinensis(L.) O.Kuntze)in Wuling Mountainous Area Revealed by ISSR Marker  

作　　者：Yue Zhang Li Lu Saiyan Yuan Xin Pang Wei-Dong Wang Lin Zhou John K Tanui Xing-Hui Li 

机构地区：[1]Tea Research Institute,Nanjing Agricultural University [2]department of tea science and biology,Wuyi College

出　　处：《茶叶》2013年第4期205-211,共7页Journal of Tea

基　　金：supported by the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-23);the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Science and Technology Program of Suzhou (SZGD201067,WZC65,WNZ1002);The PhD Gathering Program of Jiangsu;the Science and Technology Program of Jiangsu Province (BE2011319,BE2012440,BE2013426)

摘　　要：Genetic diversity and relationship of 183 tea germplasms were assessed by inter-simple sequence repeat(ISSR) marker.The study optimized the ISSR-PCR system for tea plants.Results show that genetic similarity among 183 tea ascensions was between 0.52-0.93,indicating a wide gene pool and a broad genetic variation of Wuling tea resources.The highest similarity of all materials between No1 and No2 was 0.93.Dendrogram of the183 tea germplasms were protracted by UPGMA clustering analysis also revealed the genetic diversity and relationship of the tea germplasms of Wuling mountainous area.Besides,fingerprint of the 183 tea germplasms were constructed.Genetic diversity and relationship of 183 tea germplasms were assessed by inter-simple sequence repeat （ISSR） marker.The study optimized the ISSR-PCR system for tea plants.Results show that genetic similarity among 183 tea ascensions was between 0.52-0.93,indicating a wide gene pool and a broad genetic variation of Wuling tea resources.The highest similarity of all materials between No1 and No2 was 0.93.Dendrogram of the 183 tea germplasms were protracted by UPGMA clustering analysis also revealed the genetic diversity and relationship of the tea germplasms of Wuling mountainous area.Besides,fingerprint of the 183 tea germplasms were constructed.

关 键 词：ISSR标记 遗传多样性 茶树资源 武陵山区 UPGMA聚类分析 ISSR-PCR 简单重复序列 指纹图谱构建 

分 类 号：S571.1[农业科学—茶叶生产加工]