响应面法优化柿叶总黄酮的超声波辅助提取条件  

Optimization of Ultrasonic-assisted Extraction of Flavonoids from Persimmon Leaf by Response Surface Methodology

作　　者：贾娜[1] 李晓崴[1] 宋立[1] 邵俊花[1] 赵莹莹[1] 戚军[1] 刘登勇[1] 

机构地区：[1]渤海大学食品科学研究院,辽宁省食品安全重点实验室,辽宁锦州121013

出　　处：《食品与发酵科技》2013年第6期1-6,共6页Food and Fermentation Science & Technology

基　　金：国家自然科学基金(31301509)

摘　　要：利用响应面法(Response surface methodology,RSM)优化柿叶总黄酮的超声波辅助提取条件。以提取温度、乙醇体积分数、液固比、提取时间为试验因子,以柿叶提取物中总黄酮含量为响应值,采用Box-Behnken试验设计进行试验。结果表明,提取温度对总黄酮含量影响最大,其次为液固比、乙醇体积分数和提取时间。柿叶总黄酮的最佳超声波辅助提取条件为:温度53℃、乙醇体积分数69%、液固比21∶1、提取时间32min。在此条件下,柿叶提取物总黄酮含量的预测值为35.05mg/g,验证试验所得总黄酮的含量为35.36mg/g。回归方程的预测值和试验值差异不显著,所得回归模型拟合情况良好,达到设计要求。因此,超声波辅助提取法能提高提取效率、缩短提取时间和保护有效成分,具有一定的实际应用价值。Response surface methodology （RSM） was used to optimize the experimental conditions for ultrasonic- assisted extraction of flavonoids from persimmon leaf. Box-Behnken design was employed to optimize four extraction variables including extraction temperature, ethanol concentration, ratio of aqueous ethanol to persimmon leaf and extraction time. The flavonoids content of persimmon leaf extract was the evaluation index. The results showed that the extraction temperature displayed the max impact on flavonoids content and followed by the ratio of aqueous ethanol to persimmon leaf, ethanol concentration and extraction time. The optimized conditions were： temperature 53℃, ethanol concentration 69%, ratio of aqueous ethanol to persimmon leaf 21：1, extraction time 32min. Under the optimal conditions, the predicted flavonoids content of persimmon leaf extract was 35.05mg/g, while the experimental flavonoids content of persimmon leaf extract was 35.36mg/g. The RSM-predicted yield and the experimental flavonoids content of persimmon leaf extract were not significantly different from each other. The results showed that the empirical model developed by RSM was adequate to describe the relationships between the factors and response values. The uhrasonic-assisted extraction method could improve the extraction efficiency, shorten the extraction time and protect the bioactive compounds and possess the practical application value.

关 键 词：柿叶 黄酮 超声波 提取 响应面 

分 类 号：TS201.1[轻工技术与工程—食品科学]