嗜热链球菌噬菌体的分离纯化与鉴定  

Purification and identification of Streptococcus thermophilus phage

作　　者：徐志平[1] 潘能庆[2] 徐爱才[1] 华宝珍[1] 黄丽芬[2] 马成杰[1] 

机构地区：[1]乳业生物技术国家重点实验室,光明乳业股份有限公司技术中心,上海200436 [2]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品工业科技》2014年第1期141-143,149,共4页Science and Technology of Food Industry

基　　金：国家“973”计划项目(2010CB735705)

摘　　要：噬菌体颗粒的分离纯化是研究噬菌体生物学特性、基因分析的基础工作。本研究采用聚乙二醇浓缩噬菌体增殖液、缓冲液透析去除聚乙二醇、氯化铯密度梯度离心纯化,得到了纯度较高的嗜热链球菌噬菌体样品。多重PCR鉴定和SDS-PAGE蛋白组成分析结果表明,该嗜热链球菌噬菌体的包装机制为pac-型。Phages purification is the basic work to study the characteristics and analyze the genome.A method to purify Streptococcus thermophilus phages from culture medium by means of CsCI gradient centrifugation was described.The main processing included polyethylene glycol（PEG） concentration, dialysising to remove the PEG, CsCI density gradient centrifugation and dialysising to remove the CsCI.Multiple polymerase chain reaction and Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the packaging mechanism of the phage was pac-type.

关 键 词：嗜热链球菌 噬菌体 纯化 鉴定 

分 类 号：Q939.117[生物学—微生物学]