松多酚对牛肺动脉内皮细胞抗氧化活性影响  

Antioxidant capacity of Pinus sylvestris L. polyphenlols in bovine pulmonary artery endothelial cells

作　　者：刘荣[1] 杨巍巍[1] 姜元松[1] 王振宇[1,2] 

机构地区：[1]东北林业大学林学院食品学科,黑龙江哈尔滨150040 [2]哈尔滨工业大学食品科学与工程学院生物化工系

出　　处：《中国公共卫生》2014年第1期80-82,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(31170510)

摘　　要：目的探讨松多酚对H2O2引起的牛肺动脉内皮细胞(BPAECs)氧化损伤的保护作用。方法采用H2O2诱导BPAECs建立氧化损伤模型,实验设对照组、模型组、松多酚高、中、低(0.5、0.1、0.05 mg/mL)剂量组;噻唑蓝法检测细胞存活率,试剂盒法检测抗氧化酶活性、丙二醛含量等。结果与对照组比较,模型组BPAECs存活率[(0.71±0.03)%]下降,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力[分别为(24.67±0.69)、(41.95±0.58)、(24.38±1.67)U/mg prot]下降,MDA含量[(5.83±0.04)mmol/mL]升高;与模型组比较,松多酚0.5 mg/mL组BPAECs可明显提高细胞存活率[(1.64±0.03)%],SOD、CAT和GSH-Px酶活力[分别为(53.62±1.24)、(61.01±1.10)、(48.62±0.82)U/mg prot]明显升高,MDA含量[(1.96±0.00)mmol/mL]明显降低,蛋白质羰基含量降低;呈剂量-效应关系。结论松多酚对H2O2诱导氧化损伤的BPAECs具有保护作用。Objective To investigate protective effect of Pinus sylvestris L. polyphenlols extracts on H202 induced damage in bovine pulmonary artery endothelial cells （BPAECs）. Methods Oxidative damage of BPAECs was induced by H202. The BPAECs were divided into control group, H202 model group, and high-, midium-, and low-dose poly phenlols groups （0. 5,0. 1,0. 05 mg/mL）. The influence of Pinus sylvestris L. polyphenlols extracts on H202 induced damage in BPAECs was assessed by measuring the cells＇ viability with 3-（ 4,5-dimethylthiazol-2-yl ）-2,5-diphenyltetro zolium bromide （MTT） assay. The superoxide dismutase （SOD） ; malondialdehyde （ MDA）, and glutathione peroxidase （GSH-Px） content were determined with assay kits. Results The cell viability of BPAECs decreased （0. 71 ± 0.03% ） in H202 model group compared to that of the control group. The kinases activity of SOD,catalase （CAT） ,and GSH-Px decreased （24. 67 ±0. 69,41.95 ±0. 58,24. 38 ± 1.67 U/mg/prot）. Meanwhile,the content of MDA increased （5.83 ± 0.04 mmol/mL）. In high-dose group （0. 5mg/ml） ,the cell viability of BPAECs raised remarkably （ 1.64 ± 0. 03% ） and the kinases activity of SOD, CAT, and GSH-Px improved obviously （ 53.62 ± 1.24,61.01± 1.10, and 48.62 ± 0. 82 U/mg/prot）. The contents of MDA and protein carbonyl reduced markedly （ 1.96 ± 0. 00 mmoL/mL, 1.13 ± 0.08 U/mg protein） compared to those of the H2 02 model group. The results showed a dose-effect relationship. Conclusion The Pi nus sylvestris L. polyphenlols extracts possess protective effect on H202 induced damage in EPAECs

关 键 词：松多酚 牛肺动脉内皮细胞 抗氧化活性 

分 类 号：R151.41[医药卫生—营养与食品卫生学]