适用于野生软枣猕猴桃遗传多样性分析的SSR引物筛选  

SSR Primer Screening for the Analysis of Genetic Diversity of Wild Actinitia arguta

作　　者：覃瑞[1] 赖娟娟[1] 李作洲[2] 满玉萍[2] 王彦昌[2] 

机构地区：[1]中南民族大学生命科学学院,武汉430074 [2]中国科学院武汉植物园,植物种质创新与特色农业重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2013年第4期28-32,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(3067143;31171945);农业部公益性行业农业科研专项(200903013)

摘　　要：为进一步研究野生软枣猕猴桃种质资源的遗传多样性,以软枣猕猴桃基因组DNA为模板,优化了SSR反应体系并确定了反应条件,筛选出了多态性较高的标记.从来自中华猕猴桃基因组的65对SSR引物初步筛选出15对有较好扩增带型、增稳定性好的引物,其中较好多态性的引物有12对.用筛选的引物进一步对四川天全二郎山的野生软枣猕猴桃群体进行了SSR分析,在15个个体中共检测出了141个等位基因,平均每个位点检测到9.4个等位基因,平均PIC值为0.745.In order to study the genetic diversity of wild Actinidia arguta germplasm, genome DNA of the wild spcies were used as template for deciding the SSR reaction conditions and identifying the polymorphic markers. Fifteen pairs of primers were screened out from sixty five genome SSR primers of A. chinensis, among which twelve pairs were polymorphic. 15 Actinidia samples were then collected from Erlangshan of Yaan, Sichuan, and analyzed using the above 15 primer pairs. A total of 141 allele were observed with the average number of alleles for each loci of 9.4 and the average PIC of 0.745.

关 键 词：软枣猕猴桃 简单重复序列 引物筛选 等位基因 

分 类 号：Q943[生物学—植物学]