产纤维素酶菌株Pantoea ananatis P5的筛选鉴定和活性研究  

Screening,Identification and Activity Analysis of a Cellulase Producing Strain Pantoea Ananatis P5

作　　者：侯进慧[1] 刘彤[1] 李同祥[1] 

机构地区：[1]徐州工程学院,江苏徐州221111

出　　处：《徐州工程学院学报（自然科学版）》2013年第4期80-84,共5页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

基　　金：江苏省高校自然科学基金面上项目(12KJD550003);徐州工程学院校级培育课题

摘　　要：对一种新型纤维素酶产生菌株P5进行了筛选鉴定和活性研究.该纤维素酶产生菌株在中国普通微生物保藏管理中心的保藏号是CGMCC NO.5356.利用产纤维素酶菌株筛选培养基,从土壤中筛选到一株产生纤维素酶菌株P5,对该菌株进行了分子鉴定和纤维素酶活性分析.结果显示,菌株P5分类上是Pantoea ananatis菌株,其纤维素酶适应温度和酸碱度范围较广,可应用于生物、食品、能源、纺织等领域.In the paper, screening, identification and activity analysis of a new cellulase producing strain P5 is discussed. The collection number of the strain P5 is CGMCC No. 5356. The cellulase producing strain P5 is screened and identified from soil by using sodium-carboxy-methylcellulose-containing cellulase producing strain screening and culture solution and genetic engineering technical means. The cellulase secretion characteristic of the strain indicates the cellulase secreted by the stain has the characteristics of wide temperature adaption range and certain acid and alkali resistance. The strain and the cellulase secreted by the strain have application potential in many industries, including biological energy source, cloth washing, textile and food industries.

关 键 词：纤维素酶 PANTOEA ananatis 分类 

分 类 号：Q93-331[生物学—微生物学]