响应面优化玉米芯黄酮的提取工艺研究  

Study on the extraction technology of flavonoids from corn cob by response surface methodology

作　　者：常丽新[1] 贾长虹[1] 郁春乐 

机构地区：[1]河北联合大学生命科学学院,河北唐山063009

出　　处：《食品工业科技》2014年第2期259-263,290,共6页Science and Technology of Food Industry

基　　金：河北省科技厅科技支撑项目(12227107D)

摘　　要：以乙醇溶液作溶剂,采用振荡法提取玉米芯黄酮,在单因素实验结果的基础上,根据Box-Behnken的中心组合实验设计原理,设计四因素三水平实验,利用Design-Expert软件进行响应面分析,建立了玉米芯黄酮提取的四元二次回归方程,经检验该回归方程是合理可靠的,能够较好的预测玉米芯黄酮的提取率。优化了玉米芯黄酮的最佳提取工艺条件,结果表明,乙醇浓度对黄酮提取效果影响最大,其次为样品粒度,而提取温度和料液比影响较小。最佳工艺条件为乙醇浓度85%,粒度80目,料液比1∶25(g/mL),提取温度51℃,在此条件下黄酮提取率实测值可达0.289%,实测值与理论值(0.294%)相差较小,相对误差为1.72%,充分验证了该模型的可靠性。Using ethanol as the solvent,flavonoids was extracted from the corn cob by oscillation method. Based on results of single factor experiments,using Box-Behnken central composite design,four factors three levels experiment was designed. Results were analyzed by response surface methodology with Design-Expert,and the extraction of flavonoids from corn cob was set up. The regression equation was reasonable and reliable by checking. Extraction conditions of flavonoids from the corn cob were optimized. The result showed that the main factor of influencing flavonoids extraction was ethanol concentration,next was particle size,temperature and the ratio of material to liquid was less effected. These optimum extracting conditions were 85% ethanol concentration,80 particle size,and 1∶25 ratio of material to liquid,temperature 51℃,extraction 2h,the yield of flavonoids was 0.289%. The actual measured value and the theoretical value（0.294%） of the difference was smaller,the relative error was 1.72%. The reliability of the model was proved fully.

关 键 词：玉米芯 黄酮 振荡 提取 响应面 

分 类 号：TS202.3[轻工技术与工程—食品科学]