番茄红素对鱼藤酮损伤PC12细胞的保护作用研究  

Study on protective effects of lycopene against rotenone-induced injury in PC12 cells

作　　者：罗连响[1] 李晓玲[2] 朱少平[3] 黎柔君 朱丽飞[4] 鲍波[1,3] 

机构地区：[1]广东医学院病理生理学教研室,广东湛江524023 [2]广东医学院微生物学和免疫学教研室,广东湛江524023 [3]广东医学院实验动物学教研室,广东湛江524023 [4]广东医学院第一临床医学院医学院,广东湛江524023

出　　处：《食品工业科技》2014年第2期328-331,336,共5页Science and Technology of Food Industry

基　　金：广东省科技计划项目(2010B060500015)

摘　　要：目的:探讨番茄红素(Lycopene)对鱼藤酮诱导的PC12细胞损伤的保护作用及其可能机制。方法:采用鱼藤酮诱导PC12细胞损伤模型,给予番茄红素预处理后,CCK-8法检测细胞存活率;倒置显微镜观察细胞形态,测定细胞培养液上清乳酸脱氢酶(LDH)释放量;碘化丙啶(PI)染色流式细胞术检测细胞周期变化;比色法测定细胞内谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、总抗氧化能力(T-AOC);DCFH-DA染色检测细胞内活性氧类物质(ROS)水平。结果:番茄红素能明显提高鱼藤酮损伤PC12细胞存活率(p<0.05),恢复细胞增殖;明显减轻了细胞损伤的形态的改变,降低LDH的释放、MDA和ROS含量(p<0.05),提高PC12细胞内SOD活性、GSH含量、T-AOC能力(p<0.05)。结论:番茄红素拮抗鱼藤酮致PC12细胞的损伤,其机制可能与抗氧化,清除细胞内ROS,诱导抗氧化酶的活性有关。Objective：To investigate the protective effects of lycopene against rotenone-induced injury in PC12 cells and to probe into its possible mechanisms. Methods：All experiments were carried out with PC12 in vitro, which were divided into 5 groups：control,model,lycopene（3,10,30μmol/L） preincubation. Cell viability was determined CCK-8 assay. PC12 cells were stained with PI and the percentage of different phases in the cell cycle was analyzed by flow cytometry. Then ROS production of endothelial cells in different groups was detected. Supernates and cells were collected perspectively after different treatments for measuring LDH and MDA levels,as well as GSH,SOD with the corresponding detection kit according to the manufacturers instructions. Results：Lycopene restrained the apoptotic of cells. And rescued the mitosis of damaged PC12（p0.05） and stimulated the synthesis of DNA in S-phase of cell cycle,the activity of SOD,GSH,T-AOC were increased（p0.05）. These contents of LDH,MDA and ROS were decreased（p0.05）. Conclusion：Lycopene exerted protective effects against rotenone-induced injury in PC12 cells,which might act through its anti-oxidative effects and the decrease of ROS and the induction of antioxidant enzyme activity.

关 键 词：番茄红素 鱼藤酮 PC12细胞 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]