通过紫外与伽马射线的复合诱变筛选高糖化酶活力的菌株（英文）  

UV and (60)~Co-γ-ray combined mutagenesis of Aspergillus niger for the enhancement of glucoamylase activity

作　　者：赵培城[1] 夏娟[1] 邓颜威[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《菌物学报》2014年第1期47-54,共8页Mycosystema

基　　金：Supported by the Research Initiation Fund of Zhejiang University of Technology

摘　　要：糖化酶,也称淀粉转葡萄糖酶,是淀粉水解过程中一种极其重要的酶类。通过紫外线和伽马射线的复合诱变得到一株酶活性极高的黑曲霉菌株,命名为AnUVγ‐147。该菌株的酶活性(1 182.2U/mL)是原菌株酶活性(86.5U/mL)的13.7倍,并且具有良好的稳定性,经过8代传代培养后酶活性为932.5U/mL。Glucoamylase, also known as amyloglucosidase, is an extremely important enzyme for starch hydrolysis processes. Attempts were made for higher activity of glucoamylase by a strain of Aspergillus niger （An-1） through UV and 60Co-γ ray combined mutagenesis. One prominent mutant strain designated as AnUVγ-147 variant significantly exhibited 13.7 times higher extracellular glucoamylase activity （1 182.2U/mL） as compared to the original An-1 strain （86.5U/mL）. This high expression of glucoamylase activity by the mutant was observed to be irrevocable, showing sustained stability even after eight repeated sub-culture （932.5U/mL）.

关 键 词：糖化酶 紫外 伽马射线 

分 类 号：TQ925[轻工技术与工程—发酵工程]