菜籽饼多酚的纯化  

Purification of rapeseed cake polyphenol

作　　者：熊俐[1] 曹新志[1] 谷良富 刘芳[1] 

机构地区：[1]四川理工学院生物工程学院,自贡643000

出　　处：《食品科技》2014年第1期231-235,共5页Food Science and Technology

基　　金：四川理工学院大学生创新基金项目(CX20130406)

摘　　要：从10种国产大孔树脂中筛选出XDA-1树脂对菜籽饼多酚进行纯化。通过动态吸附-解吸条件的优化研究,发现在上样量为6.25 BV、上样液pH为3.25、上样液浓度为3 mg/mL、吸附速率为1.75 BV/h的条件下,吸附率为78.64%;在解吸液(乙醇)浓度为60%、解吸液用量为5.75BV、解吸速率为1.25 BV/h、解吸液pH为3.75的条件下,解吸率为74.20%。纯化后的多酚纯度为88.32%,比多酚粗品的纯度提高了38.05%,表明XDA-1树脂对菜籽饼多酚具有良好的纯化效果。XDA-1 was selected from 10 kinds of domestic macroporous resin to purify rapeseed rake polyphenol. The conditions of dynamic adsorption-desorption were optimized here. Studies found that the adsorption percent was 78.64% under the condition of sample quantity 6.25 BV, sample liquid pH 3.25, sample liquid concentration 3 mg/mL and adsorption rate 1.75 BV/h. And the desorption percent was 74.20% under the condition of desorption solution（ethanol） concentration 60%, desorption liquid quantity 5.75 BV, desorption rate 1.25 BV/h and desorption solution pH 3.75. The purity of purified polyphenol was of 88.32%, which was increased by 38.05% compared with raw polyphenols. The result showed that XDA-1 macroporous resin had a good purification effect on rapeseed rake polyphenol.

关 键 词：菜籽饼 多酚 大孔树脂 纯化 

分 类 号：R284.2[医药卫生—中药学]