响应面法优化白骨壤多糖的辅助水提工艺  

Optimization of ultrasound-assisted extraction of polysaccharides from Avicennia marina by response surface analysis

作　　者：尹枭雄 方旭波[1,2] 余辉[1] 孙海燕[1] 王阳光[1] 

机构地区：[1]浙江海洋学院食品与医药学院,浙江舟山316004 [2]浙江省水产品加工技术研究联合重点实验室,浙江舟山316004

出　　处：《食品工业科技》2014年第3期225-229,共5页Science and Technology of Food Industry

基　　金：浙江省自然科学基金(LY12C20006)

摘　　要：对白骨壤多糖的超声提取工艺进行优化。在单因素实验的基础上,设计选取提取温度,超声时间,料液比,提取次数四个因素作为实验因素,以多糖得率为指标,利用响应面分析法,模拟得到回归方程的预估模型,最终确定白骨壤多糖的最优化提取条件为提取温度100℃,超声时间46min,料液比为1∶49,浸提次数1次,在此条件下实际得率为4.76%。In order to optimize ultrasonic extraction of polysaccharides in Avicennia marina, a mathematical regression model（ water/material ratio, extraction temperature ultrasonic time and extraction times as the factors, the yield of polysaccharides as index）for predicting extraction yield of polysaccharides was established by fitting Box-Benhnken central composite design experimental results.The optimal protocol for polysaccharides extraction from Avicennia marina was based on one time extraction for 46min at 100℃ with a ratio of water to dry material of 49：! ,the optimum extracting rate for the Avicennia marina was 4.76%.

关 键 词：超声 白骨壤 多糖 响应面分析法 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]