硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞及肿瘤细胞内游离Ca^(2+)的影响  

Effect of Selenium-Modified Exopolysaccharide from Lactococcus lactis subsp. lactis on [Ca^(2+)]_i in Mouse Macrophages and Cancer Cells

作　　者：刘鹭[1] 潘道东[1] 丁琳[1] 曾小群[1] 

机构地区：[1]宁波大学海洋学院食品系,浙江宁波315211

出　　处：《食品科学》2014年第1期250-253,共4页Food Science

基　　金：浙江省自然科学基金重点项目(Z3110211);国家自然科学基金项目(41276121);宁波市重大攻关项目(2011C11017)

摘　　要：目的:研究硒化乳酸菌胞外多糖对小鼠腹腔巨噬细胞、SGC7901胃癌细胞及海拉细胞(Hela cells)内游离Ca2+的影响。方法:分离制备小鼠腹腔巨噬细胞,利用Ca2+荧光探针Fluo-3/AM及激光共聚显微镜测定加药后10 min细胞内Ca2+浓度的变化。结果:添加硒化多糖后,巨噬细胞内Ca2+浓度缓慢升高,而胃癌细胞与Hela细胞内Ca2+水平均有不同程度的下降。结论:通过对乳酸菌胞外多糖进行硒化修饰可增强其刺激巨噬细胞的能力,因而发挥其免疫功效。硒化多糖与肿瘤细胞表面受体结合后引起细胞内Ca2+内流的减少,提示硒化后的多糖可引起肿瘤细胞不同程度的细胞凋亡。Objective： The effect of Se-exopolysaccharide （EPS） from Lactococcus lactis subsp, lactis on intracellular free Ca2＋ levels in mouse macrophages cells, gastric cancer cells SGC7901 and Hela cells was studied. Methods： Cells labeled with Fluo-3/AM dye for t0 min were examined using laser confocal scanning microscope. Results： The intracellular Ca2＋ level in mouse macrophages increased in the presence of Se-EPS. On the other hand, Se-EPS could decrease the intracellular Ca2＋ levels both in gastric cancer cells SGC7901 and Hela cells. Conclusion： Se-EPS can activate macrophages, which may be related to Ca2＊ signal pathways. Se-rich EPS has gained the capacity to induceapoptosis in tumor cells.

关 键 词：胞外多糖 硒化 巨噬细胞 钙离子 肿瘤细胞 

分 类 号：Q244[生物学—细胞生物学] Q28