考马斯亮蓝法测定核桃水溶性蛋白含量的研究  

Determination of water-soluble protein in walnut by Bradford method

作　　者：柳荫[1] 吴凤智[1] 陈龙[1] 王晓丹[1] 邱树毅[1] 周鸿翔[1] 

机构地区：[1]贵州大学酿酒与食品工程学院贵州省发酵工程与生物制药省级重点实验室,贵州贵阳550025

出　　处：《中国酿造》2013年第12期131-133,共3页China Brewing

基　　金：贵州省农业攻关项目(黔科合NZ[2013]3016号)

摘　　要：采用考马斯亮蓝G-250染色法对核桃粗蛋白中可溶性蛋白质含量进行了测定,结果表明,蛋白质浓度为10mg/mL^80mg/mL时与吸光度值有良好的线性关系,考马斯亮蓝G-250染色法简单、快速、灵敏,且相对较为准确,是测定低浓度蛋白质含量的有效方法。通过此方法,测得的核桃水溶性蛋白平均含量为8.37%。The soluble protein content in walnut was determined by using coomassie brilliant blue G-250.Result showed there was a good linear relationship between protein concentration and absorbance value within the concentration value of 10mg/mL～80mg/mL.The Bradford method was simple,rapid,sensitive and relatively accurate,which was an effective method for measuring low concentrations protein content.By this method,the measured average protein content of walnut was 8.37％.

关 键 词：核桃 蛋白质 考马斯亮蓝 

分 类 号：O657.32[理学—分析化学]