高效液相色谱法测定食品中的日落黄和柠檬黄  

Determination of sunset yellow and tartrazine in food by high performance liquid chromatography

作　　者：王宏伟[1] 于同月 李莎莎[1] 李劭彤 李巧玲[1] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《中国食品添加剂》2013年第6期190-194,共5页China Food Additives

基　　金：国家科技支撑计划(2012BAD29B04-1-1);河北省科技支撑项目(12220402D)

摘　　要：本研究以一种膨化食品香米饼(含有日落黄)和水蜜桃果汁饮料(含有日落黄和柠檬黄)作为实验材料。用溶解-离心-聚酰胺粉酸性条件下吸附-碱性条件下脱附法提取膨化食品中的色素;用稀释法来处理饮料样品。处理好后的样品,用高效液相色谱仪在梯度洗脱条件下检测两种食品中的色素含量。两种色素在2.0 40μg/mL浓度范围内线性关系良好,相对标准偏差均小于2.1%,回收率在88.3%99.9%之间,此方法精确度高、简单易行且分离效果较好。Amethod based on high performance liquid chromatography （HPLC） was developed for the determination of sunset yellow and tartrazine in puffed food and fruit beverage.Puffed foodwas dissolved by water,centrifuged by the high-speed centrifuge.The extractwas adsorbed by a polyamide SPE cartridge under acidic conditions,deadsorbed by ethanoL/ammonia/water solution （7 ∶ 2 ∶ 1,V/V） under alkali conditions.Fruit beverage was diluted by ultra-pure water.The pigments were detected by gradient elution with mobile phases of NH4Ac and methanol.The results showed that linearity of two pigments in the range 2.0 to 40.0 μg/L was good.The relative standard deviations were in the range of 0.52％ ～ 2.10％.The average recoveries ranged from 88.3％ to 99.9％ for two compounds at three different spiked levels.The method is accurate,simple and better separated for the determination of synthetic pigments infood.

关 键 词：膨化食品 饮料 日落黄 柠檬黄 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]