混菌发酵啤酒糟开发富含β-胡萝卜素饲料的研究  

Feed enrichedβ-carotene production by mixed fermentation of brewer's grains

作　　者：高娜[1] 彭鹏[1] 李瑞萍[1] 马雪[1] 张园园[1] 刘爱民[1] 

机构地区：[1]安徽师范大学∥生命科学学院生态环境与生态安全安徽省高校重点实验室∥重要生物资源的保护和利用研究安徽省重点实验室,安徽芜湖241000

出　　处：《粮食与饲料工业》2014年第2期52-55,共4页Cereal & Feed Industry

基　　金：2012年度国家级大学生创新创业训练计划项目(201210370089);2012年度安徽师范大学创新基金项目(2012cxjj04);校大学生创新性实验计划项目(2011102);培育计划项目(pyjh2012196);安徽师范大学教学研究项目(201013);省级重点实验室与省级重点学科开放基金;优秀创新团队建设计划的基金;教育部高等学校博士学科点专项科研基金(20123424110004)资助

摘　　要：研究采用毛柄金钱菌和枯草芽胞杆菌分别预处理啤酒糟后,再接种粘红酵母31229共发酵啤酒糟开发富含β-胡萝卜素的混菌饲料。结果表明:毛柄金钱菌、枯草芽胞杆菌和粘红酵母31229在啤酒糟中能够分别生长良好,粘红酵母31229能用啤酒糟等丰富而廉价的原料作为碳源生产β-胡萝卜素;以纤维素酶活力较强的毛柄金钱菌处理啤酒糟培养基5d,发酵液进行一次灭菌后再接种粘红酵母31229,4d后所得的粘红酵母31229菌体生物量和β-胡萝卜素分别达到13.10g/L和62.64μg/(g干菌体)。With Flammulina velutiper and Bacillus subtilis pretreatment of brewer＇s grain, respectively, and co-fermentation by Rhodotorula glutinis 31229, mixed bacteria enriched β--carotene feed were produced. The results showed that： Flammulina velutiper, Bacillus subtilis and Rhodotorula glutinis 31229 could grow well in brewer＇s grain. β--earotene was produced by Rhodotorula glutinis 31229 fermentation of low-priced substrate, such as brewer＇s grain. The brewer＇s grain was inoculated with high cellulase activityFlammulina velutipes for 5 d, and then inoculated with Rhodotorula glutinis 31229 after a steriliza tion, 4 days later, the biomass and carotene reached 13.10 L and 62.64 a dry cells weight, resoectivelv.

关 键 词：毛柄金钱菌 枯草芽胞杆菌 粘红酵母31229 啤酒糟 Β-胡萝卜素 发酵 

分 类 号：S816.7[农业科学—饲料科学]