高效液相色谱法测定罗非鱼中孔雀石绿残留量的不确定度  

Uncertainty Analysis in Determination of Malachite Green Residue in Oreochromis sp.by HPLC

作　　者：杨金兰[1] 李刘冬[1] 陈培基[1] 黎智广[1] 黄珂[1] 王强[1] 杨宏亮[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,农业部水产品贮藏保鲜质量安全风险评估实验室(广州),广东广州510300

出　　处：《广东海洋大学学报》2013年第6期62-66,共5页Journal of Guangdong Ocean University

基　　金：农业公益性行业科研专项(nyhyzx07-047)

摘　　要：依据JJF1059.1-2012《测量不确定度评定与表示》,分析高效液相色谱法测定罗非鱼中孔雀石绿残留量不确定度的来源,从影响不确定度的标准溶液配制us、样品前处理up和仪器测量u仪等3个主要因素,对不确定度各个分量进行计算和合成,得出罗非鱼中孔雀石绿的扩展不确定度U=0.88(k=2)。According to Measurement Uncertainty and Results＂ Indication （JJF1059.1-2012）, the sources of uncertainty in determination of malachite green residue in Oreochromis sp. by the method of HPLC were analyzed. And three factors of uncertainty, i.e. Malachite green （MG） standard solution, sample treatment and instrument measurement, were found out. Meanwhile, the uncertainty of each variable was evaluated and synthesized as well, and the result showed that the expanded uncertainty was 0.88 μg/kg （k =2）.

关 键 词：罗非鱼 孔雀石绿 药物残留 测定 不确定度 高效液相色谱法 

分 类 号：TS201.6[轻工技术与工程—食品科学]