响应面法优化红缘拟层孔菌胞外多糖发酵培养基  

Optimization of culture medium of Fomitopsis pinicola for exopolysaccharide production by response surface methodology

作　　者：圣志存 郝利民[1,2] 贾士儒[1] 陶如玉 张虹 鲁吉珂[2,4] 

机构地区：[1]天津科技大学,工业发酵微生物重点实验室,天津300457 [2]总后勤部军需装备研究所,北京100010 [3]武装警察部队总部机关门诊部,北京100089 [4]郑州大学生命科学学院,河南郑州450001

出　　处：《食品工业科技》2014年第4期194-198,203,共6页Science and Technology of Food Industry

摘　　要：为提高红缘拟层孔菌(Fomitopsis pinicola)胞外多糖(EPS)产量,采用响应面法对其发酵培养基进行优化。在单因素实验基础上,利用中心组合设计原理,以蔗糖、酵母粉、MgSO4·7H2O浓度为实验因素,以多糖产量为响应值,采用3因素5水平响应面分析方法建立数学模型。结果显示,红缘拟层孔菌产EPS的最佳培养基为:蔗糖186g/L、酵母粉7g/L,MgSO4·7H2O 27g/L,KH2PO42g/L。在此条件下,EPS产量预测值为4.02g/L,验证值为(3.92±0.18)g/L。采用响应面法对红缘拟层孔菌EPS发酵培养基进行优化,方法可行。Response surface methodology was employed to optimize the culture medium for exopolysaccharide production from Fomitopsis ptnicoe. Exopolysaccharide yield was investigated with respect to the concentration of sucrose, yeast extract and MgSO4· 7H2O based on these results of single factor experiment. A quadratic regression model was established on a three -variable, five -level center composite design, The optimum concentration of sucrose,yeast extract and MgSO4·7H2O were 186g/L,7g/L and 27g/L,resepectively, Under these conditions ,the predicted value of EPS yield was 4.02g/L,while the actual value was （3.92±0.18）g/L. So response surface methodology was feasible for optimization of fermentation medium of Fomitopsis pinico/a for EPS production.

关 键 词：红缘拟层孔菌 红缘拟层孔菌胞外多糖 响应面 

分 类 号：TS201.3[轻工技术与工程—食品科学]