Bacillus amyloliquefaciens C-1胞外发酵产物对食源性致病菌抑菌活性的初步研究  

Antimicrobial Activity on Food-Borne Pathogensof Extracellular Fermented Products from Bacillus amyloliquefaciens C-1

作　　者：周文杰[1,2] 杨建[1,2] 张瑞娟[1,2] 万茵[3] 韩蓓[1,2] 

机构地区：[1]西安交通大学医学部公共卫生学院 [2]陕西省营养与食品安全工程研究中心,西安710061 [3]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《天然产物研究与开发》2014年第1期123-127,共5页Natural Product Research and Development

基　　金：中央高校基本科研业务经费专项(xjj2012125);食品科学与技术国家重点实验室开放基金(SKLF-KF-201201)

摘　　要：对本实验室自行分离的解淀粉芽孢杆菌C-1进行发酵,离心得到其上清液,平板抑菌实验结果显示该菌上清液对蜡样芽孢杆菌MS10362R、大肠杆菌ATCC 25922及厌氧梭菌(Clostridium difficile QB21)有较强的抑菌活性;在生长曲线测定中,4 mL C-1上清液可显著延长B.cereus MS10362R细胞的迟缓期,抑制细菌生长,且可排除pH等物理因素的影响,确定了C-1发酵上清液的抑菌活性;同时发现其对于MS10362R次生代谢产物-黑色素的产生和产量具有明显的抑制作用,初步推测可能与抑制细菌的群体感应有关;而从C-1上清中提纯的胞外多糖被证明不具有抑菌活性。Bacillus amyloliquefaciens C-1 was a newly isolated strain. C-1 supernatant was collected from the 72h fermen- tation culture. It showed high antimicrobial activity against Bacillus cereus MS10362R, Clostridium di^tcile QB21and Escherichia coli ATCC25922 by culture dish assay. In growth curve analysis,4 mL of C-1 supernatant in 150mL medium inhibited the growth of B. cereus MS10362R at the lag phase, and the pH changing of culture medium by the addition of 4 mL C-1 supernatant were excluded. At the same time,the production of melanin,secondary metabolite of MS10362R, was reduced,which might be the effect of QS （quorum sensing） system. Meanwhile, exopolysaccharides extracted and purified from C-1 supernatant failed to show any antimicrobial activity.

关 键 词：解淀粉芽孢杆菌C-1 抑菌活性 胞外多糖 食源性致病菌 

分 类 号：Q939.9[生物学—微生物学]