中心组合设计优化两歧双歧杆菌BB01增殖培养基  

Optimization of enrichment media for growth of Bifidobacterium Bifidum BB01 by central composite design

作　　者：秦涛[1] 马齐[1] 舒国伟[1,2] 陈合[2] 齐欢[2] 

机构地区：[1]陕西省科学院酶工程研究所,西安710600 [2]陕西科技大学生命科学与工程学院,西安710021

出　　处：《食品科技》2014年第2期12-16,共5页Food Science and Technology

基　　金：陕西省科学院重点项目(2010K-03)

摘　　要：为了提高两歧双歧杆菌培养液中的活菌数,采用中心组合设计对其增殖培养基进行了优化。在前期单因素试验的基础上,首先通过Plackett-Burman试验筛选出了影响两歧双歧杆菌生长的4个主因子,再通过中心组合设计试验及响应面分析确定了4个主因子的最佳浓度为:三氯化铁、水苏糖、蒺藜提取液和pH分别为0.012 g/L、8.0 g/L、2.5%和6.90,用优化后的增殖培养基培养两歧双歧杆菌,24 h后其培养液OD600为1.398±0.007,比优化前提高了24.28%。In order to increase viable counts of Bifidobacterium Bifidum BB01, optimization of enrichment media was carried out by central composite design. Four most important nutrients for Bifidobacterium Bifidum BB01 were screened via Plackett-Burman using design expert. Optimal concentrations of the four factors were confirmed by central composite design and response surface analysis. The results were 0.012 g/L of FeCl 3, 8.0 g/L of stachyose, 2.50% of extract of Tribulus terrestris and pH 6.90. Cultivated with the optimized enrichment medium, the OD 600 of Bifidobacterium Bifidum BB01 after 24 hours reached 1.398±0.007, increased by 24.28% compared with the control.

关 键 词：两歧双歧杆菌 增殖培养基 中心组合设计 益生菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]