Mash1 efficiently reprograms rat astrocytes into neurons  

作　　者：Daofang Ding Leqin Xu Hao Xu Xiaofeng Li Qianqian Liang Yongjian Zhao Yongjun Wang 

机构地区：[1]Institute of Spine,Shanghai University of Traditional Chinese Medicine [2]Longhua Hospital,Shanghai University of Traditional Chinese Medicine

出　　处：《Neural Regeneration Research》2014年第1期25-32,共8页中国神经再生研究（英文版）

基　　金：supported by the National Basic Research Program of China(973 Program),No.2010CB530400;the Key Project of National Natural Science Foundation of China,No.30930111

摘　　要：To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mashl and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mashl was not detectable. Thus, we hypothesized that Mashl alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mashl. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro- nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mashl than in control cells. These results indicate that Mashl alone can reprogram astrocytes into neurons.To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mashl and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mashl was not detectable. Thus, we hypothesized that Mashl alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mashl. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro- nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mashl than in control cells. These results indicate that Mashl alone can reprogram astrocytes into neurons.

关 键 词：nerve regeneration Mash-l Brn2 Β-TUBULIN reprogram PLASMID RETROVIRUS ASTROCYTES NEURONS the National Basic Research Program of China （973 Program） neural regeneration 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]