Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes  

作　　者：Chunling Fan Hui Wang Dan Chen Xiaoxin Cheng Kun Xiong Xuegang Luo Qilin Cao 

机构地区：[1]Department of Human Anatomy and Neurobiology,Xiangya School of Medicine,Central South University [2]The Vivian L Smith Department of Neurosurgery,UT Medical School at Houston

出　　处：《Neural Regeneration Research》2014年第2期119-128,共10页中国神经再生研究（英文版）

基　　金：supported by the NIH Foundation of the USA,No.R01 NS061975;the Natural Science Foundation of Hunan Province in China,No.11JJ6077

摘　　要：The role of type-2 astrocytes in the repair of central nervous system injury remains poorly un- derstood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic pro- tein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astroc^es, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker.The role of type-2 astrocytes in the repair of central nervous system injury remains poorly un- derstood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic pro- tein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astroc^es, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker.

关 键 词：nerve regeneration spinal cord injury OLIGODENDROCYTE oligodendrocyte precursor cells ASTROCYTES bone morphogenetic protein neurons NEURITES dorsal root ganglion NIH grant neuralregeneration 

分 类 号：R741[医药卫生—神经病学与精神病学]