热带假丝酵母转化烷烃过程中P450酶活的研究  

STUDY ON THE CYTOCHROME P450 ACTIVITY IN ALKANE CONVERTING PROCESS OF CANDIDA TROPICALIS

作　　者：焦鹏[1] 华玉涛[1] 李书良[1] 黄英明[1] 曹竹安[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《微生物学报》2001年第1期117-120,共4页Acta Microbiologica Sinica

基　　金：国家"九五"科技攻关项目(96-C03-04-02);国家自然科学基金重点项目(20036010);国家自然科学基金项目(300000003、29976022);中国博士后科学基金项目,中国石油化工总公司基础研究重点项目(x598022)

摘　　要：A method of reduced CO\|difference spectrum was established to study the cytochrome P450 activity of the whole cell of \%Candida tropicalis\% during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.A method of reduced CO\|difference spectrum was established to study the cytochrome P450 activity of the whole cell of \%Candida tropicalis\% during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.

关 键 词：热带假丝酵母 烷烃 长链二元酸 细胞色素P450酶 CO差光谱 

分 类 号：TQ920.5[轻工技术与工程—发酵工程]