淡色生赤壳菌(Bionectria ochroleuca)Bo-1菌株产生抗菌物质的发酵条件优化  

Fermentation Optimization of Antimicrobial Substance Produced by Bionectria ochroleuca Strain Bo-1

作　　者：刘琴英[1] 蒋冬花[1] 齐育平[1] 孙蕾[1] 陈璨[1] 谢祥聪[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004

出　　处：《生物技术通报》2014年第1期166-170,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31270061)

摘　　要：以淡色生赤壳菌(Bionectria ochroleuca)Bo-1菌株发酵液乙酸乙酯粗提物对水稻白叶枯病菌(Xanthomonas oryzaepv.oryzae,Xoo)抑菌活性为检测指标,采用单因素试验优化Bo-1菌株产生抗菌物质培养所需的碳源、氮源、无机盐;通过正交试验优化培养基配方和摇瓶发酵条件。研究结果表明,Bo-1菌株产生抗菌物质适宜的碳源、氮源和无机盐分别为淀粉、蛋白胨、MgSO4·7H2O;优化的培养基配方为:淀粉30 g/L,蛋白胨2 g/L,MgSO4·7H2O 0.5 g/L;适宜的发酵条件为:温度30℃,转速150 r/min,装液量80 mL/250 mL,pH 6.5。Based on the index of antimicrobial activity of ethyl acetate extracts from culture liquid of Bionectria ochroleuca strain Bo-1 to Xanthomonas oryzae pv. oryzae, the optimal carbon source, nitrogen source and salt for the production of antimicrobial substance by strain Bo-1 were tested by single factor experiments. The medium composition and fermentation conditions were optimized by orthogonal experiments. The results showed that the optimal carbon source, nitrogen source and salt were starch, peptone, MgSO4.7H20, respectively. The optimal composition of the medium was 30 g/L starch, 2 g/L peptone, 0.5 g/L MgSO4. 7H20. The optimal fermentation conditions were the combination of temperature 30℃, agitation speed 150 r/min, medium volume 80 mL/250 mL, initial pH6.5.

关 键 词：淡色生赤壳菌 正交试验 发酵 优化 抑菌活性 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]