用毛细管电泳检测牦牛、犏牛和藏黄牛乳中β-乳球蛋白的三种遗传变异体  

Detection of three genetic variants of β-lactoglobulin in milk of yak,cattle-yak and Tibetan Yellow cattle by capillary electrophoresis

作　　者：任亮[1] 黄林[1] 蒋忠荣 高杰[1] 刘曦 卢帮敏[1] 金素钰[1] 林亚秋[1] 郑玉才[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]甘孜州畜牧科学研究所,四川康定626000

出　　处：《食品工业科技》2014年第5期137-140,共4页Science and Technology of Food Industry

基　　金：农业部行业专项(201203010);西南民族大学研究生创新型科研重点项目(CX2013SZ62)资助

摘　　要：利用毛细管电泳(CE)分离牛乳中β-乳球蛋白(β-Lg)的三种遗传变异体,为牛乳的质量监控提供方法。分别制备牦牛、犏牛和藏黄牛乳清,用聚丙烯酰胺凝胶电泳(PAGE)和CE分析乳清蛋白。结果表明,CE法与PAGE法都能有效分离β-Lg的三种遗传变异体;在牦牛乳中加入不同比例的犏牛乳,用CE法可检测到的犏牛乳最低添加比例约为5%,线性关系好。另外,对藏黄牛杂合型β-Lg的两种遗传变异体分析显示,CE法比PAGE法能更准确对β-Lg A和β-Lg B的相对比例进行分析。本研究结果表明,CE方法能对乳中β-Lg的三种遗传变异体进行有效分离和定量分析,在牦牛乳的质量监控方面具有潜在的应用价值。The purpose of this study was to separate three genetic variants of β-Iactoglobulin（β-Lg）in bovine milk using capillary electrophoresis （CE）, in order to provide method for milk quality control. Whey samples were prepared from whole milk of yak, cattle-yak and Tibetan Yellow cattle, and analyzed by polyacrylamide gel electrophoresis（PAGE） and CE, respectively.The results showed that both CE and PAGE could separate the three genetic variants of β-Lg;the analysis of yak milk samples added with different proportions of cattle-yak milk showed that CE method was able to detect as low as 5% of added cattle-yak milk with excellent linear relationship.In addition, the analysis of two genetic variants of β-Lg in Tibetan Yellow cattle with heterozygous genotype of β-Lg showed that CE method could more accurately determine the relative proportion of β-Lg A and β-Lg B than PAGE.The results of this study indicated that CE method could effectively separate and quantify the three genetic variants of β-Lg and has potential applications in yak milk quality control.

关 键 词：毛细管电泳 牦牛 乳 Β-乳球蛋白 遗传变异体 

分 类 号：TS207.7[轻工技术与工程—食品科学]