响应面法优化大麦苗叶绿素提取工艺  

Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

作　　者：张辉[1,2] 张娜娜[3] 马丽[1] 唐坚[3] 乔勇进[2] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]上海市农业科学院农产品保鲜加工研究中心,上海201403 [3]上海师范大学生命与环境科学学院,上海200234

出　　处：《食品科学》2014年第2期75-80,共6页Food Science

基　　金：上海市科委国际合作计划项目(073907003)

摘　　要：为优化大麦苗叶绿素的提取工艺,试验以乙醇-水为溶剂对大麦苗叶绿素进行浸渍提取。采用Plackett-Burman试验设计筛选影响大麦苗叶绿素提取量的显著因素,通过中心组合试验设计优化其提取工艺参数。结果表明:液料比、乙醇体积分数及提取时间是影响叶绿素提取量的显著因素。优化所得最佳提取工艺参数为液料比31∶1、乙醇体积分数92%、提取时间112 min。在此条件下,叶绿素提取量为13.622 mg/g,与预测值13.646 mg/g较一致。故响应面法可用于优化大麦苗叶绿素的提取工艺,且其优化工艺参数可为大麦苗叶绿素工业化生产提供参考。The extraction of chlorophyll from young barley grasses with aqueous ethanol as extraction solvent was optimized. Significant variables that influence extraction efficiency were selected by Plackett-Burman experimental design and optimized by central composite experimental design. Solid-to-solvent ratio, ethanol concentration and extraction time were identified as main influencing parameters. The optimal extraction conditions were found to be 31：1, 92% and 112 min. Under these conditions, the observed yield of chlorophyll was 13.646 mg/g, approximating to the predicted value of 13.622 mg/g. It can be concluded that response surface methodology is applicable to optimize the parameters for extraction of chlorophyll from young barley grasses and the optimized extraction process parameters can provide a reference for industrial production of chlorophyll from young barley grasses.

关 键 词：Plackett-Burman试验设计 中心组合试验设计 大麦苗叶绿素 提取工艺优化 

分 类 号：R284.2[医药卫生—中药学]