UA-SPE-HPLC快速分离法测定乳饮料中的硫氰酸钠的研究  

Ultrasonic-assisted Solid Phase Extraction for Separation and Determination of Sodium Thiocyanate in Dairy Beverage by High Performance Liquid Chromatography

作　　者：张丽媛[1] 姚笛[1] 贾鹏宇 王颖[1] 李秀波[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319 [2]黑龙江省农产品加工工程研究中心

出　　处：《黑龙江八一农垦大学学报》2014年第1期59-62,70,共5页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省教育厅科学技术研究(面上)项目(12521386)

摘　　要：建立超声辅助萃取-固相净化-高效液相色谱法测定乳饮料中硫氰酸钠的方法，重点研究了乳饮料的样品前处理方法，采用乙腈加三氯乙酸作为蛋白沉淀剂，有效实现对各类乳饮料的样品中蛋白质的沉淀和硫氰酸钠的提取。方法在0.05～50 mg·kg-1具有良好的线性关系，硫氰酸钠的日内精密度3.29%～5.56%，日间精密度4.32%～6.45%；不同乳饮料的回收率范围变化为87.73%～106.37%。此方法中硫氰酸钠检出限是0.024 mg·kg-1。Ultrasonic-assisted solid phase extraction method was developed to determine sodium thiocyanate of dairy beverage by high-performance liquid chromatography,which studied importantly the sample pretreatment. Using acetonitrile and trichloroacetic acid as precipitate protein, the precipitate protein and extraction of the sodium thiocyanate contaminant could be achieved effectively,and the calibration curve was linear in the range of 0.05-50 mg·kg-1,intra-day precision of 3.29%to 5.56%,inter-day precision of sodium thiocyanate of 4.32% to 6.45% and the recoveries of different dairy beverage range of 87.73% to 106.37%. Sodium thiocyanate of detection limits was 0.024 mg·kg-1 by this method .

关 键 词：乳饮料 超声 固相萃取 硫氰酸钠 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]