牛、羊发酵乳中多肽的筛分比较及抗炎作用初探  

Comparision and separation of types of peptides and the anti-inflammatory activities of the peptide fractions obtained from bovine & caprine fermented milk

作　　者：张悦[1] 葛武鹏[1] 贾玉良[1] 陈瑛[2] 王迁 秦立虎 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]陕西省质量技术监督局信息中心,陕西西安710006 [3]陕西杨凌质量技术检测检验所,陕西杨凌712100 [4]西安奶业科学研究所,陕西西安710065

出　　处：《食品与发酵工业》2014年第2期66-73,共8页Food and Fermentation Industries

基　　金：西北农林科技大学人才基金资助项目(Z111020923)

摘　　要：对不同菌种发酵的牛、羊发酵乳经过模拟人体消化后利用AKTA purifier 10蛋白质层析仪进行功能性多肽分离纯化及比较,同时应用小鼠肿瘤坏死因子TNF-αELISA及Western blot对所分离出的多肽进行抗炎作用分析与比较,为抗炎功能性食品的开发提供参考。结果表明:①用普通菌种发酵的牛、羊酸乳中的多肽种类少于添加了益生菌发酵的牛、羊发酵乳,普通羊酸乳中分离出多肽3种,普通牛酸乳中1种,嗜酸乳杆菌牛发酵乳中2种、羊发酵乳中4种,双歧杆菌牛发酵乳中2种、羊发酵乳中3种;②同时添加了嗜酸乳杆菌和双歧杆菌的复合菌种发酵乳中分离出了最多种类的多肽,且羊发酵乳中多于牛发酵乳;③在被测样品中小鼠肿瘤坏死因子TNF-α的浓度范围是(2.18±0.069)^(14.03±0.081)ng/L,与阳性对照(16.52±0.027)ng/L相比,所有分离出的多肽均具有一定抗炎作用;④通过比较,同时添加了嗜酸乳杆菌和双歧杆菌复合菌种的羊发酵乳中含有的一种多肽C①-2显示出最好的抗炎性能。In this study, the types of peptides purified from different strains bovine and caprine fermented milk u- sing an AKTA purifier 10 were determined after simulating human gastrointestinal digestion. The peptide fractions ob- tained from the milk samples were investigated and the differences were also evaluated. The anti-inflammatory activi- ties of the peptides purified from the milk samples （AKTA fractions） were determined using competitive TNF-a ELISA and Western blot. All above provided references for the development of the anti-inflammatory functional food. The results showed 4 aspects as follows. Firstly, the types of peptides of the bovine and caprine yoghurt fermented u- sing the traditional strains were less than those of the milk fermented using the probiotic strains. Three types of pep- tides were obtained from the caprine yoghurt and only one peptide was obtained from the bovine yoghurt. Four were from the caprine LactobaciUus acidophilus fermented milk and two were from the bovine. Three were from the caprine Bifidobacterium fermented milk and two were from the bovine. Secondly, the fermented milk using the probiotic strains Lactobacillus acidophilus and Bifidobacterium were demonstrated to possess the most types of peptides, the cap- rine fermented milk showed more compared to the bovine fermented milk. Thirdly, using the competitive TNF-a ELISA method, the mean values for the concentration of TNF-a increased from 2. 18 ± 0. 069 ng/L to 14.03 ± 0. 081 ng/L in all of the samples. Compared with the positive control 16.52 ±0. 027 ng/L, all of the peptides showed anti- inflammatory activities. Finally, based on comparison analysis of all of the samples, the greatest anti-inflammatory ac- tivity was showed by the C①-2 fraction, which was from fermented milk fermented by the probiotic strains Lactobacil- lus acidophilus and Bifidobacterium.

关 键 词：牛、羊发酵乳 多肽 抗炎性 AKTA 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]