动态监测三种儿茶素单体清除自由基能力  

Comparing the capacity of three kinds of catechins in scavenging free radicals

作　　者：刘彦霞[1] 田强[1] 

机构地区：[1]北京联合大学应用文理学院,生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《食品工业科技》2014年第6期136-139,145,共5页Science and Technology of Food Industry

基　　金：北京市教委重点实验室引导基金项目

摘　　要：以Trolox为参照物,动态监测葡萄籽中所富含的儿茶素(C)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)清除ABTS+·与DPPH·自由基的过程,比较三种儿茶素单体体外清除自由基的能力。结果显示在两种反应体系中,对于同一样品,随着浓度增大其自由基清除能力增强;Trolox与自由基反应达到平衡所需时间最短,但其自由基清除率低于ECG,两种反应体系中Trolox与EC、C清除自由基的能力大小顺序不同。总体来看,三种儿茶素单体清除自由基的能力ECG>EC>C。The in vitro scavenging capacity of （＋）-Catehicn （C）, （-）-Epicatechin （EC） and （-）-Epicatechin-3- O-gallate（ECG） were compared, by monitoring the scavenging rate of DPPH. and ABTS^＋. ,with the Trolox as the reference. The result showed that,for the same sample,the radical scavenging rate increased with its concentration increasing. Trolox reached equilibrium with the shortest time,but its ability of scavenging free radical was below that of ECG. Trolox,C,and EC were in different order in two reactive systems. In general ,the free radical scavenging capacity of these three kinds of catechins was in the order of ECG〉EC〉C.

关 键 词：儿茶素 DPPH^+ ABTS^+ 

分 类 号：TS207.3[轻工技术与工程—食品科学]