γ-聚谷氨酸发酵液预处理及提取纯化工艺  

Pretreatment and purification of poly-γ-glutamic acid from fermentation broth

作　　者：贺杨扬[1,2] 曾伟[1,2] 王青龙[1,2] 陈桂光[1,2] 梁智群[1,2] 

机构地区：[1]广西大学,生命科学与技术学院,广西南宁530004 [2]广西大学亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

出　　处：《食品工业科技》2014年第6期156-160,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(21062001);广西研究生教育创新计划资助项目(YCBZ2012004)

摘　　要：采用醇析和盐析结合的方法提取γ-PGA粗品。最佳稀释倍数为2倍,硅藻土和粉末活性炭(颗粒活性炭)用量分别定为10g/L和10g/L(15g/L),在室温下抽滤最佳pH为3.0。用5%NaCl和一倍体积乙醇提取γ-PGA,经过冷冻真空干燥可得到白色颗粒状γ-PGA粗品,提取率为95.21%,纯度可达96.89%,大大降低了乙醇用量,节约了生产成本。Abstract：By using alcohol precipitation and salting out to extract γ-PGA. The best dilution multiples was 2 times, the dosage of diatomaceous earth and powder activated carbon （granular activated carbon）, respectively, 10g/L and 10g/L（15g/L）,the best pH value was 3.0 for suction filter under the room temperature. Using 5% NaCl and double volume of ethanol for extraction of γ-PGA,by frozen vacuum drying of γ-PGA,the sample was white and granular,extraction efficiency achieved 95.21% ,purity was 96.89% ,using this method,the production input had reduced and the dosage of ethanol also highly reduced.

关 键 词：Γ-聚谷氨酸 预处理 硅藻土 活性炭 提取 

分 类 号：TS201.3[轻工技术与工程—食品科学]