丝状真菌对3-苯氧基苯甲酸降解能力及特性的研究  

Study on characteristics and ability of degradation effect of 3-phenoxybenzoic acid by filamentous fungi

作　　者：李金永[1] 刘书亮[1] 姚开[2] 李建龙[1] 

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]四川大学轻纺与食品学院,四川成都610065

出　　处：《食品工业科技》2014年第6期161-165,170,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31371775)

摘　　要：以实验室保存的产黄青霉QH、米曲霉MAY、高大毛霉MHC、黑根霉GH、啤酒酵母Y1和假丝酵母Y2等6株真菌为出发菌株,在PD培养基中振荡培养48h,QH、MAY、MHC和GH对100mg/L 3-苯氧基苯甲酸(3-PBA)的降解率分别为77.12%、77.19%、55.46%和72.38%;而Y1和Y2对3-PBA无降解。在MM培养基中振荡培养48h,6株真菌均未生长,对3-PBA无降解。14株已知霉菌和从环境中分离的12株霉菌及4株酵母菌进行3-PBA降解实验,测试的26株霉菌在PD培养基中对3-PBA均有不同程度的降解,4株酵母菌未有降解,推测丝状真菌可能具有降解3-PBA的共性。由HPLC谱图可知,不同种类霉菌降解3-PBA的产物不同,可能有不同的3-PBA降解途径与机制。此外,3-PBA对本研究所测试的丝状真菌的生长都有一定的抑制作用。Six strains saved in laboratory including Penicillium chrysogenum QH ,Aspergillus oryzae MAY,Mucor mucedo MHC, Rhizopus nigricans GH, Saccharomyces cerevisiae Y1 and Candida utilis Y2 were clutured in PD medium of 48h with oscillation. 3-phenoxybenzoic acid（100mg/L） were degraded by four mold stains 77.12%, 77.19%,55.46%,72.38% respectively. However,stains Y1 and Y2 had no degradation to 3-PBA. The six strains had no growth and no degradation to 3-PBA after oscillating clutured 48h in MM medium. Twelve strains of mold and 4 strains of yeasts isolated from environment,14 strains of known mold were tested for 3-PBA degradation,26 strains of mold had different degrees of degradation to 3-PBA and 4 strains of yeasts have no degradation in PD medium. We could speculate that filamentous fungi had the ability to degrade 3-PBA in common. Result indicated that different mold might have different 3-PBA degradation pathways and result in different degradation products showed in HPLC chromatograms. In addition,3-PBA had inhibitory effect on the growth of filamentous fungi tested in the study.

关 键 词：丝状真菌 3-苯氧基苯甲酸 降解特性 

分 类 号：Q939.5[生物学—微生物学]