双水相萃取米糠多糖工艺条件的探究  

Study on Extraction Process Conditions of Rice Bran Polysaccharide by Aqueous Two-phase System

作　　者：秦微微[1] 金婷[2] 宋学东[1] 丁振铎[1] 张衡 

机构地区：[1]黑龙江生态工程职业学院,哈尔滨150025 [2]枣庄学院生命科学系,山东枣庄277160 [3]哈尔滨市农业信息中心,哈尔滨150021

出　　处：《中国调味品》2014年第3期54-58,共5页China Condiment

基　　金：黑龙江省教育厅科技项目(12515218)

摘　　要：旨在探究聚乙二醇/硫酸铵双水相体系萃取米糠中多糖成分的最佳条件。以双水相系统的相比、分配系数、收率等为参数,探究了PEG的相对分子质量、PEG的质量分数以及硫酸铵质量分数对米糠多糖在两相系统中的分配行为的影响。结果表明:萃取最佳条件为PEG分子量为6000,硫酸铵质量分数为14.8%,PEG 6000质量分数为14.3%,多糖得率为0.826388。The aqueous two-phase system of polyethylene glycol （PEG）/ammonium sulfate for extrac-tion of polysaccharide in rice bran is studied.Use the parameters such as the volume ratio of two pha-ses,the distribution coefficient and the yield to explore the effects of the molecular weight of PEG, the mass fraction of ammonium sulfate and PEG on distribution behavior of polysaccharide of rice bran in two-phase system.It is demonstrated that the optimum conditions of extraction are as follows：the molecular weight of PEG is 6000,the mass fraction of ammonium sulfate is 14.8%,the mass fraction of PEG 6000 is 14.3%,and the yield of polysaccharide is 0.826388.

关 键 词：双水相 米糠多糖 硫酸铵 

分 类 号：TS264.2[轻工技术与工程—发酵工程]