液体培养发状念珠蓝细菌代谢物提取方法的比较分析  

Comparison of Metabolites Extracting Methods from Liquid Cultured Nostoc flagelliforme

作　　者：韩培培[1] 孙莹 段文倩 石拓[1] 贾士儒[1] 

机构地区：[1]工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2014年第1期7-10,共4页Journal of Tianjin University of Science & Technology

基　　金：国家自然科学基金资助项目(31201405;31271809);国家级"大学生创新创业训练计划"资助项目(201210057008)

摘　　要：比较了基于气相色谱–质谱联用技术(GC-MS)发状念珠蓝细菌代谢物的2种不同提取方法.在对样品进行快速洗涤和淬灭后,分别采用冷甲醇法和甲醇/氯仿法对样品进行提取,并考察了样品量对提取效果的影响.结果显示,从提取到的代谢物种类和丰度角度,冷甲醇法的提取效果要优于甲醇/氯仿法,且采用50,mg样品量的提取效果较佳.通过本方法,在发状念珠蓝细菌中共检测到46种代谢物,包括19种有机酸、1种无机酸、13种氨基酸、4种胺类、4种醇类和5种糖类物质.With gas chromatography mass spectrometry（GC-MS）,two kinds of metabolites extracting methods ofNostoc flagelliforme were compared. After being washed and quenched,samples were extracted using the cold methanol method and methanol / chloroform method,respectively. The results suggested that the better extraction effect was achieved with the cold methanol method with the 50,mg sample. Through this method,46,kinds of metabolites from Nostoc flagelliformewerede-tected and identified,including 19,kinds of organic acids,1,kind of inorganic acid,13,kinds of amino acids,4,kinds of amines,4,kinds of alcohols,and 5,kinds of sugar.

关 键 词：发状念珠蓝细菌 蓝细菌 气相色谱-质谱联用技术 代谢物 提取方法 gas CHROMATOGRAPHY mass spectrometry(GC-MS) 

分 类 号：Q93[生物学—微生物学]