海洋微生物碱性脂肪酶生产菌的分离选育  

The Isolation and Breeding of Alkaline Lipase Bacterium Strains from Sea Microbes

作　　者：杨从发[1] 王淑军[1] 陈静[1] 季方庆 孙万明 

机构地区：[1]淮海工学院食品工程系,江苏连云港222005

出　　处：《淮海工学院学报（自然科学版）》2000年第4期45-48,52,共5页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：江苏省教委自然科学指导性计划课题! (KJD:990 0 4)

摘　　要：从海水、海泥、海洋生物的消化道、碱厂土样等 43个样品中分离获得碱性脂肪酶产生菌 2 86株 ,其中 Q53菌株产酶能力最高 ,达 1 1 .4IU/m L。初步鉴定为假单孢菌落属。对研究结果进行统计分析表明 ,D/d值与 L A有线性相关关系。对 Q53脂肪酶合成影响因素的研究表明 :由尿素和玉米浆组成的有机氮源为产酶培养基的最佳氮源 ,橄榄油和豆油有利于脂肪酶的合成 ,而可溶性淀粉不利于酶的合成。产酶最适条件 :起始 p H9.5,温度 31℃ ,摇瓶转速 1 50 r/min,发酵周期 48h。alkaline lipase bacterium strains were isolated from 43 samples collected from sea water and sea soil, etc. Strain Q53, identified as pseudomonas sp., held the alkaline lipase production rate of 11.4 IU/mL with the highest activity in all these strains. Statistics and analysis showed that there was a linear interrelationship between D/d and LA . The study on the factors that affect lipase synthesis of pseudomonas Q53 showed: the organic nitrogen composed of urea and corn\|steep liquor was the best nitrogen in the medium for the strain to produce lipase; olive oil and soya oil were of benefit to lipase synthesis while soluble starch was not. The optimal conditions for lipase production were the initial pH value of 9.5, the culture temperature of 31 ℃, and the stirring rate of 150 r/min for 48 hours.

关 键 词：碱性脂肪酶 假单孢菌 海洋微生物 产酶能力 产酶培养基 摇瓶转速 发酵周期 分离选育 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]