响应面法优化杏鲍菇下脚料多糖提取工艺  

Optimization of Polysaccharide from the Residue of Pleurotus Eryngii Using Response Surface Methodology

作　　者：张庆[1] 林凯[1] 袁春红[1] 李可[1] 肖奎 向文良[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039 [2]成都榕珍菌业有限公司,四川成都611733

出　　处：《西华大学学报（自然科学版）》2014年第2期88-92,共5页Journal of Xihua University:Natural Science Edition

基　　金：四川省教育厅一般项目(13ZB0025);西华大学重点科研基金项目(Z1220531);榕珍菌业-西华大学产学研联合实验室(13205404)

摘　　要：采用热水浸提法提取杏鲍菇下脚料中的多糖类物质。通过单因素试验确定液料比、提取时间和作用温度的适宜水平,并在此基础上采用Box-Behnken响应面设计法建立影响因素的二次回归数学模型,得到杏鲍菇下脚料多糖提取的最佳工艺条件为液料比26 mL/g、提取温度87℃、提取时间3.2 h,在此条件下,杏鲍菇下脚料多糖的提取率为8.76%。Total polysaccharides were extracted from the residue of Pleurotus eryngii by hot water extraction method .One-factor-at-a-time method and response surface methodology were used to select appropriate levels of liquid -material ratio, extraction time and temperature.A quadratic regression model describing the effects of three process conditions on the extraction rate of total polysaccha -rides was developed using a Box-Behnken experimental design .The results showed that the optimal extraction conditions were deter-mined as follows：liquid/material ratio of 26 mL/g, extraction temperature 87℃and extraction time 3.2 h.Under the optimized condi-tions, the extraction rate of polysaccharide from the residue of pleurotus eryngii reached 8.76%.

关 键 词：杏鲍菇下脚料 多糖 热水浸提 响应面分析 

分 类 号：Q539[生物学—生物化学] TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]