白叶单枞黑茶多酚聚合物的分离分析及其生物活性研究(英文)  

Extraction,Analysis and Bioactivity of Dark Tea Polymeric Polyphenols Prepared from Camellia sinensis var.Baiye Dancong

作　　者：叶琼仙[1] 苗爱清[2] 程悦[1] 陈建萍[3] 王冬梅[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]广东省农业科学院茶叶研究所,广东英德513000 [3]香港大学中医药学院

出　　处：《食品科学》2014年第5期69-74,共6页Food Science

基　　金：广东省自然科学基金项目(9151064001000038);广东省大学生创新性实验计划课题(1055811179);中山大学医科学生业余科研基金项目([2012]83号)

摘　　要：利用溶剂萃取法及甲醇/丙酮溶-乙醚沉淀法对黑茶水提物进行分级分离,制备黑茶多酚聚合物(dark tea polymeric polyphenol,DPP)的5个部位,并研究其抗氧化活性及肿瘤细胞增殖抑制活性。研究测得5个DPP部位均呈弱酸性,紫外吸收特征显示其具有类似骨架结构。5个DPP均具有显著的抗氧化活性,其中3个DPP样品对结肠癌HT-29肿瘤细胞增殖具有显著抑制活性。本实验采用的甲醇/丙酮溶-乙醚沉淀法可有效分段富集黑茶中具有抗氧化及抑制肿瘤细胞增殖活性的多酚类成分。Post-fermented dark tea processes have been shown to generate mainly polymeric polyphenolic substances due to its special pile fermentation during manufacturing： Polymeric polyphenols from dark tea were fractionated and their antioxidant activity and anti-cancer capacity were investigated. Five dark tea polymeric polyphenol fractions （DPPs） were prepared from the aqueous extract of dark tea by liquid-liquid extraction, and the method of fractional precipitation employing mixtures of methanol/acetone-diethyl ether. All the DPPs exhibited a slightly acidic property and had a similar skeleton structure. They showed significant antioxidant activity and three of them had a strong inhibitory activity on proliferation of HT-29 tumor cells. The use of the fractional precipitation method employing the mixtures of methanol/ acetone-diethyl ether provided several fractions rich in phenolic components from dark tea, which exhibited significant antioxidant activity and cytotoxicity on HT-29 ceils.

关 键 词：白叶单枞黑茶 多酚聚合物 抗氧化活性 肿瘤细胞毒性 

分 类 号：TS201.2[轻工技术与工程—食品科学]