马克斯克鲁维酵母在工业生物技术中的应用  

Application Progress of Kluyveromyces marxianus in the Industrial Biotechnology

作　　者：高教琪[1] 韩锡铜[1] 孔亮[1] 袁文杰[1] 王娜[1] 白凤武[1] 

机构地区：[1]大连理工大学生命科学与技术学院,大连116024

出　　处：《中国生物工程杂志》2014年第2期109-117,共9页China Biotechnology

基　　金：国家"863"计划(2012AA021205);国家自然科学基金(21106016;21075012)资助项目

摘　　要：马克斯克鲁维酵母Kluyveromyces marxianus是目前研究较为广泛的一种非传统酵母,因其耐高温、生长速率快、底物谱广等诸多优势而越来越多地应用于工业生物技术领域。马克斯克鲁维酵母可以分泌菊粉酶、β-半乳糖苷酶等多种应用广泛的水解酶类;还可以利用菊粉类原料、乳清、糖蜜以及木糖等多种非粮底物生产乙醇;其在外源蛋白的分泌以及基因工程操作等工业分子生物学领域也取得了突破。现主要对近年来马克斯克鲁维酵母在产酶、乙醇发酵、分泌外源蛋白等诸多工业生物技术领域的研究进展及存在的挑战进行综述,为进一步推动马克斯克鲁维酵母在工业生物技术中的应用奠定基础。Kluyveromyces marxianus is a kind of ＂non-conventional＂ yeasts that have been studied widely. For its advantages of high temperature resistance, rapid growth and the capacity to utilize various substrates, there are increasing applications in the industrial biotechnology. K. marxianus is able to produce kinds of hydrolase, such as inulinase and β-galactosidase etc. And ethanol can also be achieved by K. marxianus from inulin, wheey, molasses, xylose and so on. Some advances have also been made in the molecular biology research of K. marxianus. Conseqeuntly, some significant research progresses in applications of enzyme secretion, ethanol fermentation, heterologous proteins production by K. marxianus in recent years were reviewed to facilitate further applications of K. marxianus in the industrial biotechnology.

关 键 词：马克斯克鲁维酵母 工业生物技术 菊粉酶 乙醇发酵 

分 类 号：Q939.9[生物学—微生物学]