7.0T nuclear magnetic resonance evaluation of the amyloid beta(1–40) animal model of Alzheimer's disease: comparison of cytology verification  

作　　者：Lei Zhang Shuai Dong Guixiang Zhao Yu Ma 

机构地区：[1]MR Neuroradiology Room,Beijing Tiantan Hospital Affiliated to Capital Medical University [2]Department of Neurology,Sixth People’s Hospital of Jinan [3]Department of Rehabilitation Medicine,Sixth People’s Hospital of Jinan [4]Tsinghua University Yuquan Hospital

出　　处：《Neural Regeneration Research》2014年第4期430-435,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81141013;a grant for Talents in Beijing,No.2011D003034000019

摘　　要：3.0T magnetic resonance spectroscopic imaging brain function in Alzheimer＇s disease. However, is a commonly used method in the research ot the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain function of Alzheimer＇s disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer＇s disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This finding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-field nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer＇s disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta （1-40） animal model of Alzheimer＇s disease.3.0T magnetic resonance spectroscopic imaging brain function in Alzheimer＇s disease. However, is a commonly used method in the research ot the role of 7.0T high-field magnetic resonance spectroscopic imaging in brain function of Alzheimer＇s disease remains unclear. In this study, 7.0T magnetic resonance spectroscopy showed that in the hippocampus of Alzheimer＇s disease rats, the N-acetylaspartate wave crest was reduced, and the creatine and choline wave crest was elevated. This finding was further supported by hematoxylin-eosin staining, which showed a loss of hippocampal neurons and more glial cells. Moreover, electron microscopy showed neuronal shrinkage and mitochondrial rupture, and scanning electron microscopy revealed small size hippocampal synaptic vesicles, incomplete synaptic structure, and reduced number. Overall, the results revealed that 7.0T high-field nuclear magnetic resonance spectroscopy detected the lesions and functional changes in hippocampal neurons of Alzheimer＇s disease rats in vivo, allowing the possibility for assessing the success rate and grading of the amyloid beta （1-40） animal model of Alzheimer＇s disease.

关 键 词：nerve regeneration Alzheimer＇s disease AΒ1-40 high-field functional magnetic resonance nuclear magnetic resonance spectroscopy NEUROPATHOLOGY N-ACETYLASPARTATE CREATINE CHOLINE hippocampus NSFC grant neural regeneration 

分 类 号：R749.16[医药卫生—神经病学与精神病学]