高效液相色谱法测定卤制鸡肝中两种胆固醇氧化物  

Determination of two cholesterol oxidation products in marinated chicken liver by HPLC

作　　者：李向梅[1] 周建科[1] 付艳芳[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室,河北保定071002

出　　处：《中国食品卫生杂志》2014年第2期156-159,共4页Chinese Journal of Food Hygiene

摘　　要：目的建立一种可以分析测定鸡肝中7-酮基胆固醇和胆甾烯酮的方法。方法样品与弗罗里硅土填料混合研磨后装柱，乙腈为洗脱剂，萃取与净化一步完成。无水乙醇为流动相，在240～285nm变波长条件下液相色谱测定。结果2种胆固醇氧化物在0．25～100μg／ml范围内线性关系良好，r均〉0．9993，方法检出限分别为0．015、0．045μg／g。优化条件下，组分在鸡肝样品中的平均回收率在74．1％～97．0％之间，RSDs〈5．57％。结论该方法适合于鸡肝中胆固醇氧化物的测定。Objective The aim was to establish a method for the simultaneous determination of 2 kinds of cholesterol oxidation products （COPs： 7-ketocholesterol, cholesta-4, 6-dien-3-one） by HPLC. Methods The mixture of samples and Florisil packing were grinded, then packed to a syringe column. The extraction and purification of the samples were carried out with one-step using acetonitrile as eluting reagent. With anhydrous ethanol as mobile phase, the two components were determined by liquid chromatography under 240-285 nm. Results There was a good linearity in the range of 0.25- 100 μg/ml and the correlation coefficients were greater than 0. 999 3. The detection limits for 2 COPs were 0. 015 and 0. 045μg/g, respectively. Under the optimal conditions, the recovery rates of the two target analytes were between 74. 1% - 97.0% and the relative standard deviations （RSDs） were no more than 5.57%. Conclusion This method is suitable for the determination of cholesterol oxides in chicken liver.

关 键 词：高效液相色谱 胆固醇氧化物 7-酮基胆固醇 胆甾烯酮 鸡肝 食品 

分 类 号：R155[医药卫生—营养与食品卫生学] R155.5[医药卫生—公共卫生与预防医学]