不同提取方法对印度紫檀叶片挥发性成分的GC-MS分析  

Analysis of Volatile Components from Pterocarpus indicus Leaves with Different Extraction Methods by Gas Chromatography-Mass Spectrometry

作　　者：郑丽霞[1] 高泽正[1,2] 吴伟坚[1] 梁广文[1] 符悦冠[3] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]中国科学院华南植物园,广州510520 [3]中国热带农业科学院环境与植物保护研究所,儋州571737

出　　处：《天然产物研究与开发》2014年第3期374-379,共6页Natural Product Research and Development

基　　金：公益性行业(农业)科研专项项目(201103026-4;200803 023-02);科技部农业科技成果转化资金项目(2011GB 23260037)

摘　　要：采用气相色谱-质谱法(GC-MS)分析同时蒸馏萃取法(SDE)、固相微萃取法(SPME)和动态顶空吸附法(DHA)三种方法提取的印度紫檀挥发性成分。结果表明:三种方法提取印度紫檀挥发油的化学成分有差异。同时蒸馏萃取法提取印度紫檀挥发油鉴定出25种化合物,主要成分为2-硝基乙醇,相对含量为35.29%;固相微萃取法提取印度紫檀挥发油鉴定出27种化合物,主要成分为乙酸叶醇酯,相对含量为33.62%;动态顶空吸附法提取印度紫檀挥发油鉴定出27种化合物,主要成分为2-硝基乙醇,相对含量为30.42%。三种方法共检测到2种相同组分,检出的主要物质是醇类、酯类和缩醛。The volatile components from Pterocarpus indicus leaves were extracted by simultaneous distillation extraction （SDE） ,solid phase micro-extraction （SPME） and dynamic headspace adsorption （DHA） and analyzed using gas chromatography-mass spectrometry （GC-MS）. The results indicated that the volatile oil compounds extracted out using the three methods were different. Twenty-five chemical components were identified from the SDE extract, the main component was 2-nitroethanol, accounting for 35.29% of the total identified components ;Twenty-seven chemical components were i- dentified from the SPME extract, the main component was cis-3-hexenyl-l-acetate, accounting for 33.62% of the total i- dentified components;Twenty-seven chemical components were identified from the DHA extract, the main component was 2-nitroethanol,accounting for 30.42% of the total identified components. Two compounds were detected in all the three extracts. In addition, alcohols, esters and acetals were the major components detected in all the three extracts.

关 键 词：印度紫檀 同时蒸馏萃取 固相微萃取 动态顶空吸附 气相色谱-质谱 

分 类 号：S796[农业科学—林木遗传育种] O657[农业科学—林学]