响应面法优化杜仲叶中总多酚超声波辅助提取工艺研究  

Optimization of ultrasonic assisted extraction technology for total polyphenols in leaves of Eucommia ulmoides Olive using response surface methodology

作　　者：张琳杰[1] 彭胜[1] 张昌伟[1] 王志宏[1] 周云雷[1] 彭密军[1] 张水寒[2] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,杜仲湖南省工程实验室,湖南张家界427000 [2]湖南省中医药研究院,湖南省中药新药研究与开发重点实验室,湖南长沙410013

出　　处：《食品工业科技》2014年第8期228-232,236,共6页Science and Technology of Food Industry

基　　金：国家“十二五”科技支撑计划(2011BAI01B08);湖南省科技厅项目(2012FJ4294);湖南省高校科技创新团队支持计划资助项目(湘教通(2010)212号;湖南省教育厅项目(11CY015);吉首大学研究生科研创新基金项目(JGY201226)

摘　　要：采用响应面法优化杜仲叶中总多酚超声波辅助提取工艺。考察了提取溶剂、液料比、提取时间、提取温度及提取次数对提取工艺的影响,在单因素实验分析的基础上采用Box-Benhnken中心组合进行4因素3水平的实验设计,以总多酚得率为响应值,进行响应面分析,建立二次多项回归数学模型,并优化提取工艺。结果表明杜仲叶中总多酚超声波辅助提取最佳工艺为:55%乙醇、液料比为25∶1mL·g-1、45℃下提取25min,提取两次,在此条件下,总多酚得率为4.678%。Response surface analysis methodology（ RSM） was used to optimizing the ultrasonic assisted extraction technology of total polyphenols in leaves of Eucommia ulmoides Olive. The influence on the technology caused by the variety of extraction solvents and the concentrations,liquid-to-solid ratio,extraction time,extraction temperature and extraction times was investigated. Experiments were arranged according to Box-Behnken central composite experiment design based on results of single-factor experiments. Response surface analysis was applied to create the two multinomial mathematical and optimize the extract process.Results showed that the optimum parameters for extracting from leaves of Eucommia ulmoides Olive were 55%ethanol,25 times the amount of solvent and 25min at 45℃ twice. Under these condition,the yield of total polyphenols was 4.678%.

关 键 词：杜仲叶 总多酚 超声波辅助提取 响应面法 

分 类 号：Q351[生物学—遗传学]